The significance of microRNA-184 on JAK2/STAT3 signaling pathway in the formation mechanism of glioblastoma

Oncology Letters
Xuekui ZhangX U Zhai

Abstract

Glioblastoma is a type of glioma with a relatively higher degree of malignancy that may result in severe intracranial hypertension and focal symptoms. Surgery is the preferred treatment modality. Combination therapy including radiotherapy, chemotherapy, gene therapy, immunotherapy and targeted therapy have also been employed. However, due to the invasiveness and pathogenesis of the disease, such treatments do not yield satisfactory outcomes. The aim of the present study was to examine the expression of microRNA (miR)-184 in Janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway in the mechanism of glioblastoma formation, thus providing a new basis for the mechanism of glioblastoma induction. The LN18 cell line was employed in the present study. After undergoing thawing, culturing and passaging processes, the cells were divided into the set control group, miR-184 mimic group (transfer miR-184 simulator) and miR-184 group. The expression of miR-184 was detected using quantitative polymerase chain reaction. An MTT assay was used to detect the proliferation ability of glioma cells, and clone formation ability was also detected. The cell scratch and invasion assays were used to identify the ...Continue Reading

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Citations

Jun 20, 2018·Molecular Medicine Reports·Zhenguang DuShaonian Xu
Jul 6, 2017·Oncology Reports·Xianghe LuWenfeng Li
Feb 10, 2018·Oncology Letters·Xiao-Ben WuZhi-Ming Lu
Aug 10, 2021·Journal of Clinical Laboratory Analysis·Yang XuYuhai Zhang
Aug 25, 2021·Cancer Cell International·Zheng BianXiaolu Zhang
Dec 27, 2017·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Chaojun SongZhengzheng Xiao

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Methods Mentioned

BETA
electrophoresis

Software Mentioned

SPSS

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