The significance of proline and glutamate on butanol chaotropic stress in Bacillus subtilis 168

Biotechnology for Biofuels
Gumpanat MahipantAlisa S Vangnai

Abstract

Butanol is an intensively used industrial solvent and an attractive alternative biofuel, but the bioproduction suffers from its high toxicity. Among the native butanol producers and heterologous butanol-producing hosts, Bacillus subtilis 168 exhibited relatively higher butanol tolerance. Nevertheless, organic solvent tolerance mechanisms in Bacilli and Gram-positive bacteria have relatively less information. Thus, this study aimed to elucidate butanol stress responses that may involve in unique tolerance of B. subtilis 168 to butanol and other alcohol biocommodities. Using comparative proteomics approach and molecular analysis of butanol-challenged B. subtilis 168, 108 butanol-responsive proteins were revealed, and classified into seven groups according to their biological functions. While parts of them may be similar to the proteins reportedly involved in solvent stress response in other Gram-positive bacteria, significant role of proline in the proline-glutamate-arginine metabolism was substantiated. Detection of intracellular proline and glutamate accumulation, as well as glutamate transient conversion during butanol exposure confirmed their necessity, especially proline, for cellular butanol tolerance. Disruption of the par...Continue Reading

References

Feb 1, 1981·Journal of Bacteriology·K L DeshpandeJ F Kane
Jun 23, 1995·Journal of Molecular Biology·R GardanM Débarbouillé
Sep 7, 2001·Journal of Bacteriology·A PetersohnM Hecker
Dec 18, 2001·Journal of Mass Spectrometry : JMS·J Peng, S P Gygi
Feb 2, 2002·Applied and Environmental Microbiology·Anne U Kuhlmann, Erhard Bremer
Mar 27, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·J M WoodL T Smith
Jul 19, 2002·Molecular Microbiology·Boris R Belitsky, Abraham L Sonenshein
Oct 19, 2007·Metabolic Engineering·Shota AtsumiJames C Liao
Dec 22, 2007·Extremophiles : Life Under Extreme Conditions·Susana VílchezMaximino Manzanera
Jul 29, 2008·Metabolic Engineering·Curt R FischerGregory Stephanopoulos
Dec 18, 2008·Applied Biochemistry and Biotechnology·Eric P Knoshaug, Min Zhang
May 26, 2009·Metabolic Engineering·David R NielsenKristala L Jones Prather
Sep 17, 2009·Applied Microbiology and Biotechnology·Junmei DingKeqin Zhang
Feb 2, 2010·Applied and Environmental Microbiology·Becky J RutherfordJay D Keasling
Jan 25, 2011·Microbial Biotechnology·Prashanth BhagannaJohn E Hallsworth
Apr 21, 2011·Biotechnology Advances·Sebastian TorresGuillermo R Castro
Sep 23, 2011·Biotechnology for Biofuels·Mary J Dunlop
Dec 6, 2011·Journal of Bacteriology·Susanne MosesErhard Bremer
Jun 12, 2012·Applied and Environmental Microbiology·Tamara HoffmannErhard Bremer
Sep 11, 2012·Applied Biochemistry and Biotechnology·Cindy Ng Wei TingHua Zhao
Sep 12, 2013·Biotechnology for Biofuels·Wei-Chih ChinChieh-Chen Huang
Oct 8, 2013·Trends in Biotechnology·Kyle A ZingaroEleftherios T Papoutsakis
Oct 31, 2013·Molecular Biology and Evolution·Barry G HallMiriam Barlow
Apr 5, 2015·Current Opinion in Biotechnology·Jonathan A CrayJohn E Hallsworth

❮ Previous
Next ❯

Citations

Mar 5, 2019·Metabolomics : Official Journal of the Metabolomic Society·Honglun YuanShouwen Chen
Aug 18, 2020·Applied Biochemistry and Biotechnology·David J Timson, Joshua Eardley
Nov 30, 2018·The Journal of General and Applied Microbiology·Gumpanat MahipantAlisa S Vangnai
Oct 28, 2019·MicrobiologyOpen·Nawaporn VinayavekhinWarinthorn Chavasiri
Jun 9, 2020·World Journal of Microbiology & Biotechnology·David J Timson
Aug 25, 2018·Applied Microbiology and Biotechnology·Nawaporn Vinayavekhin, Alisa S Vangnai
Aug 22, 2018·Applied Microbiology and Biotechnology·Jianmei LuoMin Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
proteomics
PCR

Software Mentioned

MASCOT
DeCyder MS differential
GraphPad InStat3

Related Concepts

Related Feeds

Biofuels (ASM)

Biofuels are produced through contemporary processes from biomass rather than geological processes involved in fossil fuel formation. Examples include biodiesel, green diesel, biogas, etc. Discover the latest research on biofuels in this feed.