The simplified two-pipette technique is more efficient than the conventional three-pipette method for blastomere biopsy in human embryos

Fertility and Sterility
S U ChenY S Yang

Abstract

To evaluate the efficiency and efficacy of a simplified two-pipette technique in comparison to the conventional three-pipette method; in the two-pipette method, a single, larger drilling/biopsy pipette is used to perform zona pellucida (ZP) drilling and blastomere aspiration for embryo biopsy. A preclinical, prospective, randomized, in vitro experiment. The reproductive unit of a university teaching hospital. Ninety-five excess embryos at the two- to four-cell stage were obtained from 35 patients undergoing IVF. At the six- to eight-cell stage, 88 embryos were allocated randomly to three groups: group I for the conventional method (n = 29), group II for the simplified technique (n = 30), and group III for controls (n = 29). The embryos then were cultured in vitro. The retrieved blastomeres were fixed and examined with fluorescence in situ hybridization using X and Y probes simultaneously. Biopsy time, successful retrieval of a blastomere, fixation of the cell, signals developed from fluorescence in situ hybridization, and growth potential and hatching capacity of the biopsied embryos were evaluated. The mean time (+/- SD) for biopsy of each embryo in group I (435 +/- 137 seconds) was significantly longer than that in group II (...Continue Reading

References

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Citations

Feb 8, 2000·Prenatal Diagnosis·J C Harper, D Wells
Sep 18, 2001·Prenatal Diagnosis·A De Vos, A Van Steirteghem
Feb 5, 2002·Journal of Medical Genetics·E Kanavakis, J Traeger-Synodinos
Dec 10, 2002·Reproductive Biomedicine Online·A MalpaniD Modi
Sep 21, 2007·Fertility and Sterility·Wei-Hua WangTraci DeSplinter
Dec 25, 2002·Acta Veterinaria Hungarica·S BodóElen Gócza
Apr 27, 2016·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·S ShinarJ Hasson
Mar 3, 2012·Clinical and Experimental Reproductive Medicine·Li-Jung ChangYu-Shih Yang
Sep 12, 2007·Reproduction in Domestic Animals = Zuchthygiene·H M Kubisch, K M Johnson
May 17, 2000·Current Opinion in Obstetrics & Gynecology·J C Harper, J D Delhanty

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