PMID: 11325533Apr 28, 2001Paper

The simulated microgravity environment maintains key metabolic functions and promotes aggregation of primary porcine hepatocytes

Biochimica Et Biophysica Acta
K J DabosJ N Plevris

Abstract

The high aspect ratio vessel allows the culture of primary porcine hepatocytes in an environment of low shear stress and simulated microgravity. Primary porcine hepatocytes have been difficult to maintain in culture long term while preserving their metabolic functions. This study was carried out in order to characterise key metabolic functions of cell aggregates formed by primary porcine hepatocytes cultured in a high aspect ratio vessel for a predetermined period of 21 days. 10(8) porcine hepatocytes were loaded into the high aspect ratio vessel and continuously rotated during the experiments. 0.7 ml of the culture medium was sampled on days 1, 2, 4, 7, 10, 14 and 21. 1H nuclear magnetic resonance spectroscopy of the culture medium, using the presaturation technique, assessed the following: glucose metabolism, glutamine synthesis and ketogenesis. There was glucose breakdown anaerobically during the first 10 days as manifested by lactate production and pyruvate and threonine consumption. After day 10 there was significantly smaller lactate production (day 1 vs day 10 P < 0.01), and significantly smaller pyruvate (day 1 vs day 14 P < 0.03) and threonine consumption (day 1 vs day 10 P < 0.002), indicative of an aerobic metabolic ...Continue Reading

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Citations

May 24, 2008·The Journal of Infectious Diseases·Cirle Alcantara WarrenRichard L Guerrant
Dec 26, 2015·Journal of Materials Science. Materials in Medicine·P R SarikaT V Kumary
Jul 9, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Geir I NedredalArthur Revhaug
Apr 25, 2012·Journal of Neuroscience Methods·Ming-Chang ChiangKuan-Hung Lin
May 18, 2016·Pharmaceutical Research·Timothy HammondHolly Birdsall
Jun 12, 2004·Cell Transplantation·Konstantinos J DabosJohn N Plevris
Aug 10, 2007·British Journal of Cancer·R A GatenbyR J Gillies
Sep 28, 2018·Molecular Plant-microbe Interactions : MPMI·Rafael Della ColettaDavid F Garvin
Mar 17, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Geir I NedredalArthur Revhaug
Jan 27, 2004·Transplantation·Konstantinos J DabosPeter C Hayes
Mar 1, 2003·European Journal of Gastroenterology & Hepatology·Konstantinos J DabosJohn N Plevris
Jun 24, 2009·Journal of Agricultural and Food Chemistry·Fabiana PiccioniLuisa Mannina

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