The simultaneous measurement of low rates of CO2 and O2 exchange in biological systems

Analytical Biochemistry
J R WillmsD B Layzell

Abstract

An instrument for measuring low rates of biological O2 exchange using an open-flow gas analysis system is described. A novel differential O2 sensor that is capable of measuring as little as 0.4 Pa O2 against a back-ground of ambient air (20,900 Pa O2), yet has a dynamic range of +/- 2000 Pa O2 (i.e., +/- ca. 2% O2) is described. Baseline drift was typically less than 0.025 Pa min-1. The differential O2 sensor was incorporated into a respiratory quotient/photosynthetic quotient analyzer that contained other environmental sensors for atmospheric pressure, absolute O2 and CO2 concentration, temperature of the differential O2 sensor block, and differential pressure between reference and sample streams. Protocols for how these sensors can be used to calibrate the differential O2 sensor and to improve its stability with time are described. Together, the differential O2 sensor, the environmental sensors, and the simple calibration techniques allow for simultaneous, noninvasive, and accurate measurements of O2 and CO2 exchange in tissues with metabolic rates as low as about 0.1 mumol O2 or CO2 h-1. Example data are provided in which O2 differentials of 3 to 41 Pa O2 were measured in an open-flow system.

References

Oct 22, 1912·The Journal of Physiology·C G Douglas, J S Haldane

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Citations

Feb 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Andrew D B LeakeyDonald R Ort
Mar 20, 2003·Plant Physiology·Yan-Ping Cen, David B Layzell
Aug 14, 2001·Plant Physiology·Y P CenD B Layzell
Mar 26, 2003·Physiologia Plantarum·Stephen Hunt
Jun 7, 2011·Environmental Pollution·F De LaenderC R Janssen
Apr 26, 2016·Journal of the Air & Waste Management Association·Nasrin Mostafavi PakDavid B Layzell
Oct 18, 2001·Journal of Experimental Botany·J S AmthorD B Layzell
Aug 12, 1999·Plant Physiology·J R WillmsD B Layzell

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