Abstract
In Sinorhizobium meliloti, choline is the direct precursor of phosphatidylcholine, a major lipid membrane component in the Rhizobiaceae family, and glycine betaine, an important osmoprotectant. Moreover, choline is an efficient energy source which supports growth. Using a PCR strategy, we identified three chromosomal genes (choXWV) which encode components of an ABC transporter: ChoX (binding protein), ChoW (permease), and ChoV (ATPase). Whereas the best homology scores were obtained with components of betaine ProU-like systems, Cho is not involved in betaine transport. Site-directed mutagenesis of choX strongly reduced (60 to 75%) the choline uptake activity, and purification of ChoX, together with analysis of the ligand-binding specificity, showed that ChoX binds choline with a high affinity (KD, 2.7 microM) and acetylcholine with a low affinity (KD, 145 microM) but binds none of the betaines. Uptake competition experiments also revealed that ectoine, various betaines, and choline derivatives were not effective competitors for Cho-mediated choline transport. Thus, Cho is a highly specific high-affinity choline transporter. Choline transport activity and ChoX expression were induced by choline but not by salt stress. Western bl...Continue Reading
References
May 7, 1976·Analytical Biochemistry·M M Bradford
Jan 30, 1991·Biochimica Et Biophysica Acta·D Le RudulierN Riou
May 1, 1991·Molecular Microbiology·T LamarkA R Strøm
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1990·Journal of General Microbiology·F Fougère, D Le Rudulier
Jan 1, 1989·Journal of Bacteriology·J A PocardD Le Rudulier
Jul 1, 1988·Journal of Bacteriology·L T SmithD Le Rudulier
Mar 1, 1986·Journal of Bacteriology·O B StyrvoldA R Strøm
Jan 1, 1985·Journal of Bacteriology·B Perroud, D Le Rudulier
Sep 1, 1984·Gene·P Prentki, H M Krisch
Jan 1, 1982·Journal of Bacteriology·H M MeadeF M Ausubel
Jun 1, 1982·Gene·A M FriedmanF M Ausubel
Jul 1, 1984·Journal of Bacteriology·T M FinanE R Signer
Jan 12, 1983·Biochimica Et Biophysica Acta·G Richarme, A Kepes
Jan 1, 1981·Molecular & General Genetics : MGG·C RosenbergF Casse-Delbart
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·G DittaD R Helinski
Sep 1, 1994·Journal of Bacteriology·J BochE Bremer
Jun 1, 1993·Microbiological Reviews·R Tam, M H Saier
Sep 1, 1996·Journal of Bacteriology·R M KappesE Bremer
Mar 1, 1997·Current Microbiology·F BrhadaD Le Rudulier
Oct 23, 1997·Journal of Bacteriology·J PlatzerR Schmitt
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·M OsteråsD Le Rudulier
Apr 27, 1999·Molecular Microbiology·R M KappesE Bremer
Jul 3, 1999·The Journal of Biological Chemistry·K E de RudderO Geiger
Sep 6, 2000·Molecular Microbiology·K E de RudderO Geiger
Jul 28, 2001·Science·F GalibertJ Batut
Apr 27, 2002·The Journal of Biological Chemistry·Keiko KashiwagiKazuei Igarashi
Jun 1, 1994·Plant Physiology·J. C. TrinchantJ. Rigaud
Jan 28, 2003·Progress in Lipid Research·Christian SohlenkampOtto Geiger
Aug 9, 2003·Molecular Plant-microbe Interactions : MPMI·Karine MandonDaniel Le Rudulier
Nov 13, 2003·The Journal of Biological Chemistry·André SchiefnerErhard Bremer
Dec 10, 2003·Microbiology·Fernando Martínez-MoralesOtto Geiger
Citations
Jan 5, 2013·Current Microbiology·Sylwia Wdowiak-WróbelWanda Małek
Apr 28, 2005·Molecular Genetics and Genomics : MGG·Jonathan D ToddAndrew W B Johnston
Feb 10, 2010·Biochemistry·Guillermo A BermejoNico Tjandra
Oct 26, 2005·The Plant Cell·Kazuyoshi TerasakaKazufumi Yazaki
Jul 4, 2006·Journal of Bacteriology·M A FoxP S Poole
Aug 23, 2006·Journal of Bacteriology·Geneviève AlloingLaurence Dupont
Jul 31, 2007·Journal of Bacteriology·Chiliang Chen, Gwyn A Beattie
Nov 4, 2008·Journal of Bacteriology·Sonja KlüsenerFranz Narberhaus
Nov 20, 2012·Journal of Bacteriology·Claudia K HerrmannDiego J Comerci
Aug 2, 2011·Journal of Bacteriology·Meriyem AktasFranz Narberhaus
Aug 1, 2009·Biological Chemistry·Christine OswaldLutz Schmitt
Mar 28, 2009·International Journal of Molecular Sciences·Christine OswaldLutz Schmitt
Aug 8, 2014·PLoS Computational Biology·Shuo GuXuhui Huang
Nov 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·T H MauchlineT M Finan
Apr 9, 2014·BMC Genomics·Steven RudderEwen Mullins
Jul 29, 2008·Current Microbiology·Zhi Jing GuSu Sheng Yang
Jul 27, 2010·European Journal of Cell Biology·Meriyem AktasFranz Narberhaus
Jul 19, 2012·Proteins·Wenlin LiNick V Grishin
Nov 19, 2009·Molecular Microbiology·Chiliang ChenGwyn A Beattie
May 26, 2010·FEMS Microbiology Reviews·Thomas EitingerErwin Schneider
Jun 11, 2011·Journal of Molecular Biology·Marco PittelkowErhard Bremer
Apr 12, 2014·Frontiers in Plant Science·Meriyem AktasFranz Narberhaus
Aug 30, 2008·FEMS Microbiology Letters·Marc A FoxPhilip S Poole
Jul 11, 2009·Molecular Plant-microbe Interactions : MPMI·Michal JasinskiMarek Figlerowicz
Dec 14, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Akifumi SugiyamaKazufumi Yazaki
Aug 26, 2019·Molecular Ecology·Leandro N LemosVictor S Pylro
Oct 19, 2017·Nature Communications·Chuandong JiaBiao Wu
Sep 10, 2008·The Journal of Biological Chemistry·Christine OswaldErhard Bremer
Nov 28, 2020·Ecotoxicology·Xin ChenChangzheng Cui
Apr 27, 2021·Computational and Structural Biotechnology Journal·Miguel A MatillaTino Krell
Dec 19, 2018·Journal of Chemical Theory and Computation·Hanif M KhanNathalie Reuter
Aug 24, 2021·Frontiers in Microbiology·Beatriz Aragón-ArandaRaquel Conde-Álvarez
Sep 22, 2021·BMC Genomics·Flavia Moura Manoel BentoFernando Luis Cônsoli