The Sinorhizobium meliloti ABC transporter Cho is highly specific for choline and expressed in bacteroids from Medicago sativa nodules

Journal of Bacteriology
Laurence DupontD Le Rudulier

Abstract

In Sinorhizobium meliloti, choline is the direct precursor of phosphatidylcholine, a major lipid membrane component in the Rhizobiaceae family, and glycine betaine, an important osmoprotectant. Moreover, choline is an efficient energy source which supports growth. Using a PCR strategy, we identified three chromosomal genes (choXWV) which encode components of an ABC transporter: ChoX (binding protein), ChoW (permease), and ChoV (ATPase). Whereas the best homology scores were obtained with components of betaine ProU-like systems, Cho is not involved in betaine transport. Site-directed mutagenesis of choX strongly reduced (60 to 75%) the choline uptake activity, and purification of ChoX, together with analysis of the ligand-binding specificity, showed that ChoX binds choline with a high affinity (KD, 2.7 microM) and acetylcholine with a low affinity (KD, 145 microM) but binds none of the betaines. Uptake competition experiments also revealed that ectoine, various betaines, and choline derivatives were not effective competitors for Cho-mediated choline transport. Thus, Cho is a highly specific high-affinity choline transporter. Choline transport activity and ChoX expression were induced by choline but not by salt stress. Western bl...Continue Reading

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Citations

Jan 5, 2013·Current Microbiology·Sylwia Wdowiak-WróbelWanda Małek
Nov 20, 2012·Journal of Bacteriology·Claudia K HerrmannDiego J Comerci
Aug 2, 2011·Journal of Bacteriology·Meriyem AktasFranz Narberhaus
Mar 28, 2009·International Journal of Molecular Sciences·Christine OswaldLutz Schmitt
Nov 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·T H MauchlineT M Finan
Jul 27, 2010·European Journal of Cell Biology·Meriyem AktasFranz Narberhaus
Jul 19, 2012·Proteins·Wenlin LiNick V Grishin
Jun 11, 2011·Journal of Molecular Biology·Marco PittelkowErhard Bremer
Apr 12, 2014·Frontiers in Plant Science·Meriyem AktasFranz Narberhaus
Aug 30, 2008·FEMS Microbiology Letters·Marc A FoxPhilip S Poole
Jul 11, 2009·Molecular Plant-microbe Interactions : MPMI·Michal JasinskiMarek Figlerowicz
Dec 14, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Akifumi SugiyamaKazufumi Yazaki
Apr 27, 2021·Computational and Structural Biotechnology Journal·Miguel A MatillaTino Krell
Dec 19, 2018·Journal of Chemical Theory and Computation·Hanif M KhanNathalie Reuter

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