The sinus venosus myocardium contributes to the atrioventricular canal: potential role during atrioventricular node development?

Journal of Cellular and Molecular Medicine
Tim P KelderMonique R M Jongbloed

Abstract

The presence of distinct electrophysiological pathways within the atrioventricular node (AVN) is a prerequisite for atrioventricular nodal reentrant tachycardia to occur. In this study, the different cell contributions that may account for the anatomical and functional heterogeneity of the AVN were investigated. To study the temporal development of the AVN, the expression pattern of ISL1, expressed in cardiac progenitor cells, was studied in sequential stages performing co-staining with myocardial markers (TNNI2 and NKX2-5) and HCN4 (cardiac conduction system marker). An ISL1+/TNNI2+/HCN4+ continuity between the myocardium of the sinus venosus and atrioventricular canal was identified in the region of the putative AVN, which showed a pacemaker-like phenotype based on single cell patch-clamp experiments. Furthermore, qPCR analysis showed that even during early development, different cell populations can be identified in the region of the putative AVN. Fate mapping was performed by in ovo vital dye microinjection. Embryos were harvested and analysed 24 and 48 hrs post-injection. These experiments showed incorporation of sinus venosus myocardium in the posterior region of the atrioventricular canal. The myocardium of the sinus ven...Continue Reading

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Citations

Feb 10, 2016·Differentiation; Research in Biological Diversity·Tim P KelderMonique R M Jongbloed
Nov 20, 2016·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·Demosthenes G KatritsisJack Rickard
May 19, 2018·Pediatric Cardiology·Rajiv A MohanVincent M Christoffels
Nov 10, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Tim P KelderAdriana C Gittenberger-de Groot
Mar 22, 2015·The Journal of Biological Chemistry·Tim P KelderMonique R M Jongbloed
Oct 23, 2016·Cellular and Molecular Life Sciences : CMLS·Xingqun LiangYunfu Sun
Nov 4, 2017·Journal of Cardiovascular Development and Disease·David S Park, Glenn I Fishman
Jan 26, 2018·Journal of Cardiovascular Development and Disease·Rajiv MohanVincent M Christoffels
Feb 13, 2021·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Minna HassinenMatti Vornanen
Mar 7, 2021·International Journal of Molecular Sciences·Flavio L RonzoniMaurilio Sampaolesi
Mar 16, 2019·The American Journal of Cardiology·Dora L GyenesLisa K Hornberger
May 1, 2021·Journal of Cardiovascular Development and Disease·Joshua C PetersonMarco C DeRuiter
Jul 3, 2021·Journal of Cardiovascular Development and Disease·Mathilde R RivaudBastiaan J Boukens

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Methods Mentioned

BETA
dissection
PCR
Laser capture microdissection
laser capture microscopy

Software Mentioned

ImageJ
Adobe Photoshop
AMIRA
RoboSoftware
pClamp10

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