PMID: 9428008Jan 15, 1998Paper

The site and time of expression of MIF in frog development

Pigment Cell Research
T FukuzawaM Nishioka

Abstract

A ventrally localized melanization-inhibiting factor (MIF) has been suggested to play a role in the expression of dorsal-ventral pigment patterns in amphibia. Here we investigate the onset and localization of MIF appearance in frog development. The expression of MIF was analyzed in the wild-type and gray-eyed mutant (g/g) of Rana japonica by immunoblotting and immunohistochemistry using an anti-MIF neutralizing monoclonal antibody. Western blot analysis revealed that the anti-MIF antibody recognized approximately 51 kDa and approximately 58 kDa bands. The 51 kDa band first appeared at the external gill stage, while 58 kDa band was additionally detected at the hindlimb bud stage. With the use of immunohistochemistry, it was found that the anti-MIF antibody stained the whole epidermis of the embryos at the external gill stage; however, the staining was stronger in lateral and ventral epidermis than in dorsal. Staining with the anti-MIF antibody was observed only in the outer epidermis of the ventral skin, but not in the dorsal skin during and after metamorphosis. The spatial expression of MIF in the wild-type was the same as that in the gray-eyed mutant. The same immunohistochemical result was obtained in the adults of R. nigroma...Continue Reading

References

Feb 2, 1979·Science·J T BagnaraJ D Taylor
Dec 24, 1992·Cell·S J BultmanR P Woychik
Sep 1, 1988·Developmental Biology·T Fukuzawa, H Ide
Feb 1, 1984·Canadian Journal of Genetics and Cytology. Journal Canadien De Génétique Et De Cytologie·J B Armstrong
Aug 1, 1994·Differentiation; Research in Biological Diversity·C PéchouxL Frappart
Jan 1, 1994·Annual Review of Genetics·I J Jackson
Jun 7, 1994·Proceedings of the National Academy of Sciences of the United States of America·H VrielingG S Barsh
Aug 1, 1996·Trends in Genetics : TIG·G S Barsh
Jan 1, 1946·The Anatomical Record·A C TAYLOR, J J KOLLROS

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