The SK channel blocker apamin inhibits slow afterhyperpolarization currents in rat gonadotropin-releasing hormone neurones.

The Journal of Physiology
Masakatsu KatoYasuo Sakuma

Abstract

Gonadotropin-releasing hormone (GnRH) neurones play an essential role in the hypothalamo-pituitary-gonadal axis. As for other neurones, the discharge pattern of action potentials is important for GnRH neurones to properly function. In the case of a luteinizing hormone (LH) surge, for example, GnRH neurones are likely to continuously fire for more than an hour. For this type of firing, GnRH neurones must have a certain intrinsic property. To address this issue, we investigated the voltage-gated Ca(2+) currents and Ca(2+)-activated voltage-independent K(+) currents underlying afterhyperpolarization, because they affect cell excitability. Dispersed GnRH neurones from adult GnRH-EGFP (enhanced green fluorescent protein) transgenic rats were cultured overnight and then used for an electrophysiological experiment involving the perforated patch-clamp configuration. The GnRH neurones showed five subtypes of voltage-gated Ca(2+) currents, i.e. the T-, L-, N-, P/Q- and R-types. The GnRH neurones also showed a slow afterhyperpolarization current (I(sAHP)), but not a medium one. It is reported that the SK channel blocker apamin (10 pm-100 nm) blocks a medium afterhyperpolarization current but not an I(sAHP). In contrast to previous reports...Continue Reading

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Citations

Aug 6, 2009·Cell Biochemistry and Biophysics·E S Louise Faber
Jan 27, 2010·The Journal of Physiological Sciences : JPS·Nobuyuki TanakaMasakatsu Kato
Sep 8, 2010·Brain Research·Oline K RønnekleivChunguang Zhang
Aug 24, 2007·Clinical and Experimental Pharmacology & Physiology·E S Louise Faber, Pankaj Sah
Jun 8, 2012·Endocrinology·Garrett T Gaskins, Suzanne M Moenter
Sep 29, 2011·Annual Review of Physiology·John P AdelmanPankaj Sah
Dec 1, 2007·The Journal of Physiology·Melissa L PalmerScott M O'Grady
Jul 27, 2010·Brain Research·Yong Wang, M Cathleen Kuehl-Kovarik
May 20, 2011·Journal of Neuroendocrinology·O K RønnekleivC Zhang
Oct 6, 2010·Brain Research·Suzanne M Moenter
Jan 12, 2013·Molecular and Cellular Endocrinology·Martha A BoschOline K Rønnekleiv
Mar 23, 2013·Channels·Rachael NorbergKelly J Suter
Apr 10, 2009·Biology of Reproduction·Miho WatanabeMasakatsu Kato
Jan 8, 2008·The Journal of Physiological Sciences : JPS·Yoshie HiraizumiMasakatsu Kato
Jun 20, 2019·Frontiers in Molecular Neuroscience·Csaba VastaghZsolt Liposits
Apr 4, 2013·American Journal of Physiology. Endocrinology and Metabolism·Chunguang ZhangMartin J Kelly
Jun 15, 2021·EMBO Molecular Medicine·Alberto CataneseTobias M Böckers

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