The small airway epithelium as a target for the adverse pulmonary effects of silver nanoparticle inhalation

Nanotoxicology
Chang GuoMartin O Leonard

Abstract

Experimental modeling to identify specific inhalation hazards for nanomaterials has in the main focused on in vivo approaches. However, these models suffer from uncertainties surrounding species-specific differences and cellular targets for biologic response. In terms of pulmonary exposure, approaches which combine 'inhalation-like' nanoparticulate aerosol deposition with relevant human cell and tissue air-liquid interface cultures are considered an important complement to in vivo work. In this study, we utilized such a model system to build on previous results from in vivo exposures, which highlighted the small airway epithelium as a target for silver nanoparticle (AgNP) deposition. RNA-SEQ was used to characterize alterations in mRNA and miRNA within the lung. Organotypic-reconstituted 3D human primary small airway epithelial cell cultures (SmallAir) were exposed to the same spark-generated AgNP and at the same dose used in vivo, in an aerosol-exposure air-liquid interface (AE-ALI) system. Adverse effects were characterized using lactate, LDH release and alterations in mRNA and miRNA. Modest toxicological effects were paralleled by significant regulation in gene expression, reflective mainly of specific inflammatory events. I...Continue Reading

References

Jun 13, 2006·Current Problems in Dermatology·Alan B G Lansdown
Apr 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Isao NakaoKenji Izuhara
Jul 22, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yasuhiro NakagamiDavid J Erle
Sep 2, 2008·American Journal of Respiratory Cell and Molecular Biology·Anna C ZemkeBarry R Stripp
Oct 28, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alberto IzzottiSilvio De Flora
Nov 27, 2008·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jae Hyuck SungIl Je Yu
Feb 25, 2009·ACS Nano·P V AshaRaniSuresh Valiyaveettil
Sep 8, 2009·Environmental Science & Technology·Koji KawataSatoshi Okabe
Apr 30, 2010·Archives of Toxicology·Rasmus FoldbjergHerman Autrup
Jun 4, 2011·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Alice LimoncielPaul Jennings
Jun 23, 2011·Analytical and Bioanalytical Chemistry·Michaela AufderheideDieter Hochrainer
Oct 4, 2011·Environmental Health Perspectives·Milan SrivastavaWilliam T Self
Nov 22, 2011·Toxicology Letters·Christiane BeerHerman Autrup
Jul 27, 2012·Toxicological Sciences : an Official Journal of the Society of Toxicology·Rasmus FoldbjergChristiane Beer
Aug 29, 2012·Archives of Toxicology·Paul JenningsMartin O Leonard
Sep 4, 2012·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Porntipa ChairuangkittiRawiwan Maniratanachote
Dec 15, 2012·American Journal of Respiratory Cell and Molecular Biology·Stefan H GorissenAlbert van der Vliet
Feb 5, 2013·Toxicological Sciences : an Official Journal of the Society of Toxicology·Brandi N Snyder-TalkingtonNancy L Guo
Oct 3, 2013·The Journal of Clinical Investigation·Anca DorhoiStefan H E Kaufmann
Oct 26, 2013·Experimental Biology and Medicine·Cheng-Teng NgLin-Yue Lanry Yung
Nov 16, 2013·Regulatory Toxicology and Pharmacology : RTP·Niels Hadrup, Henrik R Lam
Nov 22, 2013·Epidemiology·Serena FossatiJoel Schwartz
Jan 1, 2014·The Journal of Allergy and Clinical Immunology·Carina MalmhällMadeleine Rådinger
Jun 2, 2014·Biomaterials·Magdiel Inggrid SetyawatiDavid Tai Leong
Sep 18, 2014·Particle and Fibre Toxicology·Hedwig M BraakhuisMargriet Park
Sep 28, 2014·Colloids and Surfaces. B, Biointerfaces·Kristen K ComfortSaber M Hussain
Dec 30, 2014·Journal of Toxicology·Dwaipayan MukherjeePanos G Georgopoulos
Jan 13, 2015·Toxicological Sciences : an Official Journal of the Society of Toxicology·Donald S AndersonLaura S Van Winkle
Jan 16, 2016·Toxicological Sciences : an Official Journal of the Society of Toxicology·Jennifer D SislerYong Qian
May 18, 2016·Colloids and Surfaces. B, Biointerfaces·Sinbad SweeneyTeresa D Tetley
Oct 28, 2016·Scientific Reports·Giulia GorrieriLuis J V Galietta
Nov 20, 2016·The Journal of Biological Chemistry·Shoichi SuzukiKenji Izuhara

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.