PMID: 9188617Jul 1, 1997Paper

The small envelope protein is required for secretion of a naturally occurring hepatitis B virus mutant with pre-S1 deleted

Journal of Virology
M MelegariJ R Wands

Abstract

Naturally occurring deletions in the hepatitis B virus pre-S1 domain have been frequently found during persistent viral infection. In this study we have investigated the functional properties of a mutant viral genome that carries an in-frame deletion of 183 nucleotides in the pre-S1 region. This deletion removes the promoter of the small envelope gene. Transfection into human hepatocellular carcinoma cells of a replication-competent construct containing this deletion resulted in an increase of intermediate DNA replicative forms compared to those produced by wild-type hepatitis B virus. Northern blot analysis revealed that such cells lack the 2.1-kb transcripts encoding the small envelope protein and that hepatitis B surface antigen was absent as well. Furthermore, nucleocapsids containing the genome with pre-S1 deleted were not secreted, and the deleted large envelope protein was retained with the cytoplasm and exhibited a perinuclear pattern of distribution. However, coexpression with the small envelope protein was sufficient to restore virion secretion and to change the cellular distribution of the deleted large envelope protein. In addition, the creation of point mutations that prevent the synthesis of large or small envelop...Continue Reading

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Citations

Feb 28, 2006·Journal of Virology·Philip MeulemanGeert Leroux-Roels
Jan 25, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Teresa PollicinoGiovanni Raimondo
Mar 26, 2004·Journal of Medical Virology·Bing-Fang ChenPei-Jer Chen
Jan 30, 2004·Molecular and Cellular Biology·Gilad Doitsh, Yosef Shaul
Mar 29, 2006·Journal of Gastroenterology·Takeshi Okanoue, Masahito Minami
Jun 9, 2012·Liver International : Official Journal of the International Association for the Study of the Liver·Jia-Horng KaoBing-Fang Chen
Oct 16, 2014·Journal of Biomedical Science·Ih-Jen SuWenya Huang
Jun 25, 2015·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R A A Pondé
Sep 28, 2016·World Journal of Gastroenterology : WJG·Bing-Fang Chen
May 11, 2002·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·Antonella GrottolaErica Villa
Jun 4, 2005·Alimentary Pharmacology & Therapeutics·F FabriziP Martin
May 11, 2002·Journal of Cellular and Molecular Medicine·C Kreutz
Oct 7, 2008·World Journal of Gastroenterology : WJG·Tao ShenHong Dong
Nov 30, 2012·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Robério A A Pondé
Apr 8, 2016·World Journal of Gastroenterology : WJG·Hui-Lan ZhuZhen-Hua Zhang
May 12, 2020·Alimentary Pharmacology & Therapeutics·Daniela CavalloneMaurizia Rossana Brunetto
Oct 31, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M SterneckH Will
Mar 21, 2003·Pediatric Research·Patrick GernerStefan Wirth
Feb 5, 2002·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Petra PreikschatHelga Meisel
May 19, 2005·The Journal of Biological Chemistry·Tsurng-Juhn HuangAi-Li Shiau
Sep 10, 1999·Reviews in Medical Virology·S L NguiC G Teo
Jan 22, 2010·Archives of Virology·R A A PondéM O Ferro
Apr 13, 2011·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R A A Pondé
Dec 15, 2020·Reviews in Medical Virology·Huizhen SunLunan Wang
Dec 29, 2020·AIMS Microbiology·Manoj Kumar Rajput
Apr 18, 2018·World Journal of Gastroenterology : WJG·Bing-Fang Chen
Feb 9, 2012·Journal of Biomedical Science·Chih-Ming LinBing-Fang Chen
Sep 19, 2021·Gut·Dina KremsdorfPatrick Soussan

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