PMID: 9635436Jul 11, 1998Paper

The small GTP-binding protein RhoA regulates a delayed rectifier potassium channel

Cell
T G CacheroE G Peralta

Abstract

Tyrosine kinases activated by G protein-coupled receptors can phosphorylate and thereby suppress the activity of the delayed rectifier potassium channel Kv1.2. Using a yeast two-hybrid screen, we identified the small GTP-binding protein RhoA as a necessary component in this process. Coimmunoprecipitation experiments confirmed that RhoA associates with Kv1.2. Electrophysiological analyses revealed that overexpression of RhoA markedly reduced the basal current generated by Kv1.2 expressed in Xenopus oocytes. Furthermore, in 293 cells expressing Kv1.2 and ml muscarinic acetylcholine receptors, inactivating RhoA using C3 exoenzyme blocked the ability of ml receptors to suppress Kv1.2 current. Therefore, these results demonstrate that RhoA regulates Kv1.2 activity and is a central component in the mechanism of receptor-mediated tyrosine kinase-dependent suppression of Kv1.2.

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M PaulmichlD E Clapham
Feb 25, 1987·Nucleic Acids Research·P YeramianA Tavitian
Jun 1, 1995·Trends in Biochemical Sciences·Y TakaiH Nakanishi
Aug 4, 1995·European Journal of Pharmacology·T YamagishiN Taira
Feb 1, 1995·Current Opinion in Genetics & Development·A J Ridley
Jan 1, 1994·Annual Review of Cell Biology·A Hall
Jan 1, 1994·Annual Review of Biochemistry·C Nuoffer, W E Balch
Feb 1, 1994·Current Opinion in Genetics & Development·C Nobes, A Hall
Dec 16, 1993·Nature·M S Boguski, F McCormick
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·X Y HuangE G Peralta
Jan 1, 1993·Cellular Signalling·S Narumiya, N Morii
Jun 1, 1996·Current Opinion in Neurobiology·E A Jonas, L K Kaczmarek
May 1, 1996·Trends in Biochemical Sciences·M Symons
Feb 1, 1997·Current Opinion in Cell Biology·N Tapon, A Hall
Feb 24, 1997·FEBS Letters·J KellerK H Jakobs
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·N MeiriD L Alkon

❮ Previous
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Citations

Dec 6, 2005·Pflügers Archiv : European journal of physiology·Todd M Rossignol, S V Penelope Jones
Jun 11, 2008·Pflügers Archiv : European journal of physiology·Anatoli LvovIlana Lotan
Aug 9, 2011·Pflügers Archiv : European journal of physiology·Helene Vacher, James S Trimmer
Mar 12, 2009·Molecules and Cells·Hyun Dong Je, Uy Dong Sohn
May 4, 2013·Journal of Bioenergetics and Biomembranes·Nihal OzturkSemir Ozdemir
Aug 10, 2001·Current Opinion in Structural Biology·D L Minor
Dec 17, 2003·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·H-S MoonS-C Kim
Jun 25, 2004·Molecular Biology of the Cell·Edmund NestiAnthony D Morielli
May 1, 2009·Molecular Biology of the Cell·Lee StirlingAnthony D Morielli
Jan 24, 2004·Journal of Experimental Botany·Isabelle Chérel
May 31, 2003·Journal of Cardiovascular Pharmacology·Atsushi SugiyamaKeitaro Hashimoto
Feb 18, 2005·Acta Pharmacologica Sinica·Jun Ren, Cindy X Fang
Jun 29, 2000·Annual Review of Biochemistry·K KaibuchiM Amano
Jun 3, 2000·Annual Review of Pharmacology and Toxicology·V P SahJ H Brown
Jun 22, 1999·The Journal of Clinical Investigation·T Finkel
Sep 27, 2001·Proceedings of the National Academy of Sciences of the United States of America·B A YiL Y Jan
May 25, 2013·Experimental Brain Research·Maria Cristina D'AdamoMauro Pessia
Nov 19, 2015·Journal of Cellular Physiology·Gaofeng ZhangBing Song
May 3, 2011·Progress in Neurobiology·Chen Gu, Joshua Barry
Mar 15, 2008·Journal of Molecular and Cellular Cardiology·Frank Lezoualc'hEric Morel
Jul 25, 2009·The EMBO Journal·Amy GuilfoyleMika Jormakka
May 29, 2003·The Journal of Urology·Ryosuke TakahashiHideo Kanaide
Oct 22, 2008·Journal of Cellular Physiology·Stephanie B BoyerS V Penelope Jones
Oct 31, 2000·Neuron·B A Yi, L Y Jan
Jan 16, 2002·Current Biology : CB·Nina M StoreyDavid L Armstrong
Feb 25, 2014·Neuron·Jeffrey M DonleaGero Miesenböck
Oct 26, 2007·Experimental Biology and Medicine·Oleh PochynyukAlexander Staruschenko
Apr 20, 1999·The Journal of Cell Biology·Y FukataK Kaibuchi
Jul 12, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Ben-Gang WangQian Xu
Jun 28, 2018·Journal of Neurophysiology·Ileana Hernández-AraizaTamara Rosenbaum
Apr 1, 2006·Circulation Research·Joan Heller BrownMark A Sussman
Jan 18, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jun RenPiero Anversa
Dec 6, 2002·American Journal of Physiology. Cell Physiology·Jaladanki N RaoJian-Ying Wang
Oct 19, 2013·Physiological Reviews·Gervaise LoirandPierre Pacaud
Oct 9, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Atsuko YataniLei Wei
Nov 1, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Kevin D LuykenaarDonald G Welsh
Oct 25, 2000·The Journal of Biological Chemistry·M Ichida, T Finkel

❮ Previous
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