The small GTPase ARL2 is required for cytokinesis in Trypanosoma brucei.

Molecular and Biochemical Parasitology
Helen P PriceDeborah F Smith

Abstract

The Arf-like (Arl) small GTPases have a diverse range of functions in the eukaryotic cell. Metazoan Arl2 acts as a regulator of microtubule biogenesis, binding to the tubulin-specific chaperone cofactor D. Arl2 also has a mitochondrial function through its interactions with BART and ANT-1, the only member of the Ras superfamily to be found in this organelle to date. In the present study, we describe characterization of the Arl2 orthologue in the protozoan parasite Trypanosoma brucei. Modulation of TbARL2 expression in bloodstream form parasites by RNA interference (RNAi) causes inhibition of cleavage furrow formation, resulting in a severe defect in cytokinesis and the accumulation of multinucleated cells. RNAi of TbARL2 also results in loss of acetylated alpha-tubulin but not of total -tubulin from cellular microtubules. While overexpression of TbARL2(myc) also leads to a defect in cytokinesis, an excess of untagged protein has no effect on cell division, demonstrating the importance of the extreme C-terminus in correct function. TbARL2 overexpressing cells (either myc-tagged or untagged) have an increase in acetylated -tubulin. Our data indicate that Arl2 has a fundamentally conserved role in trypanosome microtubule biogenesi...Continue Reading

References

Feb 1, 1987·The Journal of Cell Biology·G PipernoX J Chang
Jan 1, 1988·Biology of the Cell·J M Gallo, E Precigout
May 9, 1986·Cell·M Kirschner, T Mitchison
Jan 1, 1997·Annual Review of Cell and Developmental Biology·A Desai, T J Mitchison
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·H NgôE Ullu
Sep 17, 1999·The Journal of Biological Chemistry·J D Sharer, R A Kahn
Nov 23, 2000·Molecular and Biochemical Parasitology·D J LaCountJ E Donelson
Sep 8, 2001·Protist·D J LaCount, J E Donelson
Jan 26, 2002·Molecular Biology of the Cell·J Daniel SharerRichard A Kahn
May 25, 2002·Nature·Charlotte HubbertTso-Pang Yao
Jan 17, 2003·Nature·Alexander PalazzoGregg G Gundersen
Apr 23, 2003·Molecular and Biochemical Parasitology·Seth RedmondMark C Field
Aug 13, 2003·The Journal of Biological Chemistry·Jack F ShernRichard A Kahn
Dec 20, 2003·Nature Reviews. Molecular Cell Biology·Stefan Westermann, Klaus Weber
Dec 8, 2004·Experimental Parasitology·Annelise SahinGilles Merlin
Dec 22, 2004·Molecular and Biochemical Parasitology·Vincent P AlibuDavid Horn
Mar 19, 2005·Molecular Microbiology·Tansy C HammartonJeremy C Mottram
Jul 16, 2005·Science·Matthew BerrimanNajib M El-Sayed
Oct 29, 2005·Science·Cynthia Y HeGraham Warren
Nov 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Jason CarnesKenneth Stuart
Dec 24, 2005·Molecular Biology of the Cell·Anne Fourest-LieuvinDidier Job
Mar 10, 2006·Molecular Biology of the Cell·Chengjing ZhouRichard A Kahn
Mar 28, 2006·The American Journal of Pathology·Jeffrey J SchrickDennis S Rice
Dec 16, 2006·Molecular and Biochemical Parasitology·Elena MillsDeborah F Smith
Dec 22, 2006·Molecular Biology of the Cell·Helen P PriceDeborah F Smith
Apr 25, 2007·The Journal of Biological Chemistry·J Bradford BowzardRichard A Kahn
May 18, 2007·Annual Review of Cell and Developmental Biology·Alison K Gillingham, Sean Munro

❮ Previous
Next ❯

Citations

Jun 10, 2011·Cellular and Molecular Life Sciences : CMLS·Ewa Szolajska, Jadwiga Chroboczek
May 19, 2011·Nature Reviews. Molecular Cell Biology·Julie G Donaldson, Catherine L Jackson
Apr 2, 2015·Critical Reviews in Biochemistry and Molecular Biology·Helen R Mott, Darerca Owen
Dec 14, 2011·Topics in Companion Animal Medicine·Robin B Gasser, Cinzia Cantacessi
Jun 25, 2013·Parasite Immunology·L MacLeanH P Price
May 3, 2013·Clinical Science·Enzo R Porrello
Oct 12, 2018·PloS One·Gabriela T NiemirowiczLeón A Bouvier
Sep 26, 2017·Cellular Logistics·Laura E NewmanRichard A Kahn
Dec 12, 2019·Frontiers in Cellular and Infection Microbiology·Tansy C Hammarton
Sep 1, 2019·Trends in Parasitology·Amy N Sinclair, Christopher L de Graffenried
Mar 30, 2021·Frontiers in Cellular and Infection Microbiology·Victoria Lucia AlonsoEsteban Serra
Jun 19, 2021·European Journal of Protistology·Jean de Oliveira SantosMaria Cristina M Motta

❮ Previous
Next ❯

Methods Mentioned

BETA
GTPase
GTPases
flow cytometry
PCR
confocal
transfection
scanning electron microscopy
flow
confocal microscopy
acetylation

Software Mentioned

LSM 510
BLAST
Primer Express
Sequence Detection
Summit
RNAit

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.