The small GTPase R-Ras regulates organization of actin and drives membrane protrusions through the activity of PLCepsilon

Journal of Cell Science
Aude S Ada-NguemaPatricia J Keely

Abstract

R-Ras, an atypical member of the Ras subfamily of small GTPases, enhances integrin-mediated adhesion and signaling through a poorly understood mechanism. Dynamic analysis of cell spreading by total internal reflection fluorescence (TIRF) microscopy demonstrated that active R-Ras lengthened the duration of initial membrane protrusion, and promoted the formation of a ruffling lamellipod, rich in branched actin structures and devoid of filopodia. By contrast, dominant-negative R-Ras enhanced filopodia formation. Moreover, RNA interference (RNAi) approaches demonstrated that endogenous R-Ras contributed to cell spreading. These observations suggest that R-Ras regulates membrane protrusions through organization of the actin cytoskeleton. Our results suggest that phospholipase Cepsilon (PLCepsilon) is a novel R-Ras effector mediating the effects of R-Ras on the actin cytoskeleton and membrane protrusion, because R-Ras was co-precipitated with PLCepsilon and increased its activity. Knockdown of PLCepsilon with siRNA reduced the formation of the ruffling lamellipod in R-Ras cells. Consistent with this pathway, inhibitors of PLC activity, or chelating intracellular Ca2+ abolished the ability of R-Ras to promote membrane protrusions and ...Continue Reading

References

Jun 1, 1994·The Biochemical Journal·M SpaargarenJ R Bischoff
Apr 5, 1996·Cell·Z ZhangE Ruoslahti
Feb 1, 1996·Biochemical Society Transactions·E J Pettit, M B Hallett
Jul 17, 1997·International Journal of Cancer. Journal International Du Cancer·J R SpoonsterE C Kohn
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·S L JonesE J Brown
Sep 9, 1999·The Journal of Cell Biology·A Mallavarapu, T Mitchison
Sep 10, 1999·The Journal of Biological Chemistry·Y van KooykC G Figdor
Nov 24, 1999·The Journal of Biological Chemistry·H Q SunH L Yin
Dec 22, 1999·The American Journal of Physiology·A S LaderH F Cantiello
Jan 12, 2000·The Journal of Cell Biology·D Raucher, M P Sheetz
Jun 1, 2000·Cell Adhesion and Communication·L YueJ Li
Jun 23, 2000·The Journal of Biological Chemistry·K M LinH L Yin
Sep 9, 2000·Current Opinion in Cell Biology·Y van Kooyk, C G Figdor
Oct 7, 2000·The Journal of Biological Chemistry·C SongT Kataoka
Feb 17, 2001·The EMBO Journal·G G KelleyA V Smrcka
May 31, 2001·Science·D PantaloniM F Carlier
Feb 23, 2002·Trends in Cell Biology·J Victor SmallKlemens Rottner
Dec 10, 2002·Annual Review of Physiology·Helen L Yin, Paul A Janmey
Jan 28, 2003·Biochemical Society Transactions·J L BosF Zwartkruis
Jan 29, 2003·Proceedings of the National Academy of Sciences of the United States of America·John L TanChristopher S Chen
Feb 5, 2003·The Journal of Cell Biology·Tatyana M SvitkinaGary G Borisy
Feb 15, 2003·The Journal of Biological Chemistry·Werner J H KoopmanPeter H G M Willems
Feb 26, 2003·Cell·Thomas D Pollard, Gary G Borisy
Mar 19, 2003·The Journal of Cell Biology·Danijela VignjevicGary G Borisy
Apr 19, 2003·Proceedings of the National Academy of Sciences of the United States of America·Naoki ItanoErkki Ruoslahti
May 2, 2003·Proceedings of the National Academy of Sciences of the United States of America·Jan CzyzykKim Bottomly

❮ Previous
Next ❯

Citations

Jan 8, 2008·Naunyn-Schmiedeberg's Archives of Pharmacology·Sara S RoscioniMartina Schmidt
Jun 15, 2011·Medical Oncology·Xiaoliang WangZhihai Peng
Nov 7, 2008·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Paolo P ProvenzanoPatricia J Keely
Mar 13, 2013·Immunology and Cell Biology·Prithu Sundd, Klaus Ley
Dec 21, 2007·The New England Journal of Medicine·Attila PatocsCharis Eng
Jul 31, 2008·The Journal of Biological Chemistry·Kedar S VaidyaDanny R Welch
Jul 2, 2011·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Qin WangChristopher A McCulloch
Nov 16, 2011·Biochemistry. Biokhimii︠a︡·S V Shirshev
Feb 20, 2010·BMC Cell Biology·Matthew W ConklinPatricia J Keely
Oct 14, 2011·Viruses·Sofia V ZaichickGregory A Smith
Apr 3, 2012·Annual Review of Biochemistry·Emi Mizuno-YamasakiPeter Novick
Aug 21, 2010·Canadian Journal of Physiology and Pharmacology·Victoriya A RufanovaAndrey Sorokin
Aug 13, 2011·Journal of Gastroenterology·Dewei LuoZhengdong Zhang
Sep 13, 2006·The Journal of Cell Biology·Lawrence E GoldfingerMark H Ginsberg
Sep 12, 2008·Kidney International·Victoriya A RufanovaAndrey Sorokin
Dec 20, 2011·Critical Reviews in Oncology/hematology·Shawn P CareyCynthia A Reinhart-King
Jul 15, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Xue YangChunli Luo
Apr 7, 2009·Advances in Enzyme Regulation·Mario J RebecchiJonathan D Stallings
Jul 3, 2015·Scientific Reports·Ulrike MayTero A H Järvinen
May 11, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Yun-Zhao ChenFeng Li
Jul 18, 2012·Asian Pacific Journal of Cancer Prevention : APJCP·Hasnan JaafarMogana Das Murtey
Apr 17, 2014·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Jinhan WangNaiyao Chen
Jun 26, 2015·Journal of Cancer Research and Clinical Oncology·Rui-Yan ZhangDong-Sheng Pei
Mar 4, 2011·Bioscience Reports·Vegesna RadhaKotagiri Sasikumar
May 13, 2017·Immunity, Inflammation and Disease·Laura KummolaIlkka S Junttila
Jul 22, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Li ZhaoLiang Ma
Apr 9, 2015·Journal of Neuropathology and Experimental Neurology·Duthika M MallawaaratchyKimberley L Kaufman
Feb 20, 2018·Critical Reviews in Biochemistry and Molecular Biology·Saeideh Nakhaei-RadMohammad Reza Ahmadian
Jun 7, 2006·The Journal of Biological Chemistry·Maider López De JesúsMartina Schmidt
Nov 30, 2018·Journal of Cellular Biochemistry·Mengru ZengLi Xiao
Nov 3, 2016·American Journal of Physiology. Cell Physiology·Pin LvMei Han
May 29, 2020·G3 : Genes - Genomes - Genetics·Xinyan WangYu Chung Tse
Jan 17, 2021·The Journal of Biological Chemistry·Hironori InabaTakao Nakata

❮ Previous
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