The small noncoding DsrA RNA is an acid resistance regulator in Escherichia coli

Journal of Bacteriology
R A LeaseM Belfort

Abstract

DsrA RNA is a small (87-nucleotide) regulatory RNA of Escherichia coli that acts by RNA-RNA interactions to control translation and turnover of specific mRNAs. Two targets of DsrA regulation are RpoS, the stationary-phase and stress response sigma factor (sigmas), and H-NS, a histone-like nucleoid protein and global transcription repressor. Genes regulated globally by RpoS and H-NS include stress response proteins and virulence factors for pathogenic E. coli. Here, by using transcription profiling via DNA arrays, we have identified genes induced by DsrA. Steady-state levels of mRNAs from many genes increased with DsrA overproduction, including multiple acid resistance genes of E. coli. Quantitative primer extension analysis verified the induction of individual acid resistance genes in the hdeAB, gadAX, and gadBC operons. E. coli K-12 strains, as well as pathogenic E. coli O157:H7, exhibited compromised acid resistance in dsrA mutants. Conversely, overproduction of DsrA from a plasmid rendered the acid-sensitive dsrA mutant extremely acid resistant. Thus, DsrA RNA plays a regulatory role in acid resistance. Whether DsrA targets acid resistance genes directly by base pairing or indirectly via perturbation of RpoS and/or H-NS is n...Continue Reading

References

Jan 1, 1990·Methods in Enzymology·M BelfortP S Chandry
Jan 1, 1988·Annual Review of Genetics·R W Simons, N Kleckner
Apr 1, 1980·Journal of Molecular Biology·M J Casadaban, S N Cohen
Mar 14, 1995·Proceedings of the National Academy of Sciences of the United States of America·D Sledjeski, S Gottesman
Jan 1, 1993·Infection and Immunity·J Gorden, P L Small
Apr 1, 1997·Molecular Microbiology·T Atlung, H Ingmer
Sep 5, 1997·Science·F R BlattnerY Shao
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·R A LeaseM Belfort
Oct 15, 1998·Proceedings of the National Academy of Sciences of the United States of America·N MajdalaniS Gottesman
Mar 9, 1999·Trends in Microbiology·K M WassarmanG Storz
May 29, 1999·Journal of Bacteriology·M P Castanie-CornetJ W Foster
Sep 11, 1999·Nucleic Acids Research·C S RichmondF R Blattner
Dec 11, 1999·Nucleic Acids Research·K E Rudd
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·V SperandioJ B Kaper
Jan 7, 2000·Journal of Molecular Biology·K S Gajiwala, S K Burley
Aug 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·R A Lease, M Belfort
Aug 30, 2000·Proceedings of the National Academy of Sciences of the United States of America·S Altuvia, E G Wagner
Dec 15, 2000·Molecular Microbiology·R A Lease, M Belfort
Feb 27, 2001·Journal of Bacteriology·D D SledjeskiA Zhang
Mar 17, 2001·Molecular Microbiology·N MajdalaniS Gottesman
Jun 8, 2001·Journal of Bacteriology·F Repoila, S Gottesman
Apr 5, 2002·Advances in Genetics·E Gerhart H WagnerPascale Romby
Nov 16, 2002·Current Opinion in Genetics & Development·Thomas S Whittam, Alyssa C Bumbaugh
Nov 19, 2002·Genes & Development·Susan Gottesman
Apr 16, 2003·Molecular Microbiology·Nobuhisa Masuda, George M Church
May 6, 2003·Journal of Bacteriology·Don L TuckerTyrrell Conway
Sep 17, 2003·Genes & Development·Eric MasséSusan Gottesman

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Citations

Mar 19, 2008·Applied Microbiology and Biotechnology·Zhen KangQingsheng Qi
Nov 16, 2011·Biotechnology Letters·Zhen KangQingsheng Qi
Nov 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Kai PapenfortJörg Vogel
Nov 23, 2010·The Journal of Antimicrobial Chemotherapy·Kunihiko NishinoAkihito Yamaguchi
Feb 1, 2007·Nucleic Acids Research·Johannes H Urban, Jörg Vogel
Jul 31, 2013·Nucleic Acids Research·Stefan M GaidaEleftherios T Papoutsakis
Apr 12, 2011·Journal of Bacteriology·Yirong SunHiroshi Kobayashi
Jul 4, 2006·Journal of Bacteriology·Amy M JonesThomas Elliott
May 11, 2010·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Boyu Zhao, Walid A Houry
Aug 24, 2007·PLoS Biology·Erel LevineTerence Hwa
Jun 14, 2013·PloS One·Rafael A TesoreroKyu Hong Cho
Apr 8, 2014·PloS One·Rajnikant SharmaRanjana Pathania
Jan 1, 2012·ISRN Microbiology·Lorraine T Stauffer, George V Stauffer
Feb 13, 2014·Applied Microbiology and Biotechnology·Zhen KangJian Chen
Oct 9, 2014·Nucleic Acids Research·Anat Lavi-ItzkovitzErel Levine
Apr 9, 2005·Critical Reviews in Biochemistry and Molecular Biology·Nadim MajdalaniSusan Gottesman
Sep 18, 2009·Current Opinion in Microbiology·Cynthia Mira Sharma, Jörg Vogel
Oct 22, 2008·Current Opinion in Microbiology·Erel Levine, Terence Hwa
Jun 19, 2007·Current Opinion in Microbiology·Jörg Vogel, E Gerhart H Wagner
May 7, 2010·Molecular Microbiology·Matthias GimpelSabine Brantl
Nov 25, 2004·Journal of Molecular Biology·Richard A Lease, Sarah A Woodson
Apr 19, 2006·International Journal of Medical Microbiology : IJMM·Maria-Halima LaaberkiFrancis Repoila
Apr 1, 2006·Biochemical and Biophysical Research Communications·Yong ZhangRunsheng Chen
Aug 12, 2014·Tuberculosis·Tatiana KondratievaAlexander Apt
Mar 20, 2010·FEMS Microbiology Reviews·Juana María Navarro LlorensEsteban Martínez-García
May 3, 2015·FEMS Microbiology Reviews·Kai Papenfort, Carin K Vanderpool
Dec 30, 2008·Molecular Cell·Marie BouvierJörg Vogel
Jan 20, 2012·ACS Synthetic Biology·Vandana SharmaYohei Yokobayashi
Nov 9, 2016·Frontiers in Microbiology·Bram VivijsChris W Michiels
Jan 10, 2017·ACS Synthetic Biology·Ashwin LahiryRichard A Lease
Nov 16, 2017·Microbial Cell Factories·Alejandro Negrete, Joseph Shiloach
Nov 5, 2017·Biochemical Society Transactions·Erik Holmqvist, E Gerhart H Wagner

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