The Small RNA ErsA of Pseudomonas aeruginosa Contributes to Biofilm Development and Motility through Post-transcriptional Modulation of AmrZ

Frontiers in Microbiology
Marilena FalconeGiovanni Bertoni

Abstract

The small RNA ErsA of Pseudomonas aeruginosa was previously suggested to be involved in biofilm formation via negative post-transcriptional regulation of the algC gene that encodes the virulence-associated enzyme AlgC, which provides sugar precursors for the synthesis of several polysaccharides. In this study, we show that a knock-out ersA mutant strain forms a flat and uniform biofilm, not characterized by mushroom-multicellular structures typical of a mature biofilm. Conversely, the knock-out mutant strain showed enhanced swarming and twitching motilities. To assess the influence of ErsA on the P. aeruginosa transcriptome, we performed RNA-seq experiments comparing the knock-out mutant with the wild-type. More than 160 genes were found differentially expressed in the knock-out mutant. Parts of these genes, important for biofilm formation and motility regulation, are known to belong also to the AmrZ transcriptional regulator regulon. Here, we show that ErsA binds in vitro and positively regulates amrZ mRNA at post-transcriptional level in vivo suggesting an interesting contribution of the ErsA-amrZ mRNA interaction in biofilm development at several regulatory levels.

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Citations

Apr 20, 2018·MSphere·David A BaltrusRachel Murillo
Nov 30, 2018·International Journal of Molecular Sciences·Tiago PitaJorge H Leitão
Jun 27, 2019·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth M Melson, Melissa M Kendall
Oct 28, 2019·Frontiers in Cellular and Infection Microbiology·Shubham Chakravarty, Eric Massé
Aug 28, 2021·International Journal of Molecular Sciences·Petra PusicUdo Bläsi

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Datasets Mentioned

BETA
E-MTAB-6247

Methods Mentioned

BETA
RNA-seq
PCR
Scanning Microscopy
electrophoresis
electrophoretic mobility shift assay

Software Mentioned

HTSeq count
Tecan
ImageQuant
HTSeq package
IMARIS
Magellan
COMSTAT
ArrayExpress
IntaRNA
BWA aligner

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