PMID: 9443964Mar 14, 1998Paper

The small subunits of human and mouse DNA polymerase epsilon are homologous to the second largest subunit of the yeast Saccharomyces cerevisiae DNA polymerase epsilon

Nucleic Acids Research
M JokelaJ E Syväoja

Abstract

Human DNA polymerase epsilon is composed of a 261 kDa catalytic polypeptide and a 55 kDa small subunit of unknown function. cDNAs encoding the small subunit of human and mouse DNA polymerase epsilon were cloned. The predicted polypeptides have molecular masses of 59.469 and 59.319 kDa respectively and they are 90% identical. The human and mouse polypeptides show 22% identity with the 80 kDa subunit of the five subunit DNA polymerase epsilon from the yeast Saccharomyces cerevisiae. The high degree of conservation suggests that the 55 kDa subunit shares an essential function with the yeast 80 kDa subunit, which was earlier suggested to be involved in S phase cell cycle control in a pathway that is able to sense and signal incomplete replication. The small subunits of human and mouse DNA polymerase epsilon also show homology to the C-terminal domain of the second largest subunit of DNA polymerase alpha. The gene for the small subunit of human DNA polymerase epsilon (POLE2) was localized to chromosome 14q21-q22 by fluorescence in situ hybridization.

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Citations

Mar 24, 1999·Trends in Biochemical Sciences·M MäkiniemiJ E Syväoja
Sep 13, 1994·Proceedings of the National Academy of Sciences of the United States of America·A BlankL A Loeb
Dec 5, 1995·Proceedings of the National Academy of Sciences of the United States of America·H ArakiA Sugino
Jun 5, 2002·Annual Review of Biochemistry·Ulrich HubscherSilvio Spadari
Nov 19, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Toshiaki TsubotaHisaji Maki
May 14, 2009·Bioscience Reports·Erin N McMurrayJennifer V Schmidt
Apr 24, 2001·Environmental and Molecular Mutagenesis·A Ronen, B W Glickman
Jun 8, 2001·The Journal of Biological Chemistry·M MäkiniemiJ E Syväoja

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