The smaller, the better? The size effect of alginate beads carrying plant growth-promoting bacteria for seed coating

Journal of Microencapsulation
Teresa BerningerClaudia Preininger

Abstract

A range of lab-scale methods for encapsulation of plant growth-promoting bacteria in alginate beads intended for seed coating was evaluated: contact-spotting, extrusion through syringe with/without vibration, ejection by robotic liquid handler, extrusion by centrifugal force and commercial devices (nanodispenser, aerodynamically assisted jetting, encapsulator). Two methods were selected based on throughput (encapsulator: 1.5-5 mL/min; syringe with subsequent pulverisation: 5 mL/min). Four bead sizes (55 ± 39 μm, 104 ± 23 μm, 188 ± 16 μm and 336 ± 20 μm after lyophilisation) were produced. Bacterial viability, release, bead morphology, seed surface coverage and attrition were investigated. Release from the smallest bead size was approximately 10 times higher than from the largest. Seed surface coverage was highest (69 ± 3%) when alginate beads produced with nozzle size 80 μm were applied. Pulverised macro-beads are an alternative option, if high throughput is top priority.

References

Jun 6, 2000·Advanced Drug Delivery Reviews·S Wee, W R Gombotz
Jan 5, 2002·Advanced Drug Delivery Reviews·Allan S Hoffman
Feb 10, 2004·Bioresource Technology·Ghasem NajafpourKu Syahidah Ku Ismail
Apr 19, 2006·Biotechnology and Bioengineering·Chiu-Chung YoungA B Arun
Apr 25, 2006·Journal of Microbiological Methods·C A MorganG Vesey
Oct 1, 2010·Critical Reviews in Biotechnology·Rojan P JohnDanielle Prévost
Nov 26, 2011·Journal of Biosciences·Armando Hernández García
Nov 30, 2011·Progress in Polymer Science·Kuen Yong Lee, David J Mooney
Mar 1, 2012·Journal of Microencapsulation·Mauricio SchoebitzDenis Poncelet
Aug 30, 2012·Nature Methods·Caroline A SchneiderKevin W Eliceiri
Mar 20, 2013·World Journal of Microbiology & Biotechnology·R WiwattanapatapeeM Kanjanamaneesathian

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Citations

Oct 24, 2020·International Journal of Biological Macromolecules·Jishma PanichikkalRadhakrishnan Edayileveetil Krishnankutty

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