The smallest ssDNA phage infecting a marine bacterium

Environmental Microbiology
Yuanchao Zhan, Feng Chen

Abstract

In the marine environment, only a few lytic single-stranded DNA (ssDNA) phages have been isolated and characterized, despite the fact that diverse ssDNA bacteriophages have been discovered via metagenomic studies. In this study, we isolated and characterized a new ssDNA phage, vB_RpoMi-Mini, which infects a marine bacterium Ruegeria pomeroyi DSS-3. With a genome size of 4248 bp and only four putative open reading frames (ORF), vB_RpoMi-Mini becomes the smallest ssDNA phage among the known ssDNA phage isolates and represents the DNA bacteriophage with the least number of ORFs. Genome-wide analysis reveals that bacteriophage Mini is distantly related to the known ssDNA phages and belongs to an unclassified ssDNA phage within the Microviridae family. The presence of peptidase in vB_RpoMi-Mini genome further implies that horizontal gene transfer could be an important driving force in the evolution of ssDNA phages. Bacteriophage Mini seems to have lost the spike protein commonly seen in ssDNA phages, suggesting that ssDNA phage can be more diverse than previously thought. Metagenomic analysis indicates that Mini-like phages are widely distributed in the environments. The discovery of vB_RpoMi-Mini expands our understanding of ssDNA ...Continue Reading

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Citations

Feb 27, 2020·ELife·Paul C Kirchberger, Howard Ochman
Dec 18, 2018·Environmental Microbiology·Yuanchao Zhan, Feng Chen
Feb 12, 2021·Molecular Biology and Evolution·Jorge A Moura de SousaEduardo P C Rocha
May 15, 2021·Microbiology Resource Announcements·Simona KrabergerArvind Varsani
Jul 11, 2021·Proceedings of the National Academy of Sciences of the United States of America·Paul C KirchbergerHoward Ochman

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