The SMC5/6 Complex Interacts with the Papillomavirus E2 Protein and Influences Maintenance of Viral Episomal DNA

Journal of Virology
Peris BentleyPeter M Howley

Abstract

The papillomavirus E2 protein executes numerous essential functions related to viral transcription, replication of viral DNA, and viral genome maintenance. Because E2 lacks enzymatic activity, many of these functions are mediated by interactions with host cellular proteins. Unbiased proteomics approaches have successfully identified a number of E2-host protein interactions. We have extended such studies and have identified and validated the cellular proteins structural maintenance of chromosome 5 (SMC5) and SMC6 as interactors of the viral E2 protein. These two proteins make up the core components of the SMC5/6 complex. The SMC5/6 complex is a member of the conserved structural maintenance of chromosomes (SMC) family of proteins, which are essential for genome maintenance. We have examined the role of SMC5/6 in various E2 functions. Our data suggest that SMC6 is not required for E2-mediated transcriptional activation, E1/E2-mediated transient replication, or differentiation-dependent amplification of viral DNA. Our data, however, suggest a role for SMC5/6 in viral genome maintenance.IMPORTANCE The high-risk human papillomaviruses (HPVs) are the etiological cause of cervical cancer and the most common sexually transmitted infect...Continue Reading

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Citations

Jul 10, 2019·Annual Review of Virology·Tami L Coursey, Alison A McBride
May 28, 2019·Frontiers in Oncology·Tarik Gheit
Jul 11, 2020·Cell·Xing LiuJuan I Fuxman Bass
Apr 14, 2021·Nucleic Acids Research·Stephen T HallettAntony W Oliver
Apr 13, 2021·Frontiers in Immunology·Kinjal Majumder, Abigail J Morales
Oct 19, 2019·Trends in Microbiology·Alessandra De LeoPaul M Lieberman

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