The SMN interactome includes Myb-binding protein 1a

Journal of Proteome Research
H R FullerG E Morris

Abstract

Understanding networks of interacting proteins is a major goal in cell biology. The survival of motor neurons protein (SMN) interacts, directly or indirectly, with a large number of other proteins and reduced levels of SMN cause the inherited disorder spinal muscular atrophy (SMA). Some SMN interactions are stable and stoichiometric, such as those with gemins, while others are expected to be transient and substoichiometric, such as the functional interaction of SMN with coilin in Cajal bodies. This study set out to determine whether novel components of the extensive SMN interactome can be identified by a proteomic approach. SMN complexes were immuno-precipitated from HeLa nuclear extracts, using anti-SMN monoclonal antibody attached to magnetic beads, digested with trypsin, separated by capillary-liquid chromatography and analyzed by MALDI TOF/TOF mass spectrometry. One-hundred and one proteins were detected with a p value of <0.05, SMN, gemins and U snRNPs being the dominant "hits". Sixty-nine of these were rejected after MALDI analysis of two control pull-downs using antibodies against unrelated nuclear proteins. The proteins found only in anti-SMN pulldowns were either known SMN partners, and/or contained dimethylated RG dom...Continue Reading

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Citations

Dec 14, 2012·Proceedings of the National Academy of Sciences of the United States of America·Meng-Tzu WengJi Luo
Dec 12, 2012·Nucleic Acids Research·David PiñeiroEncarnación Martínez-Salas
Mar 5, 2014·The Journal of Clinical Investigation·Thomas M WishartThomas H Gillingwater
Aug 26, 2014·Assay and Drug Development Technologies·Jonathan J CherryJill Jarecki
Oct 19, 2010·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Ruben J Cauchi
Feb 16, 2012·The EMBO Journal·Chang-Ching YangBertrand Chin-Ming Tan
Aug 9, 2011·Neuromuscular Disorders : NMD·Emma HumphreyGlenn E Morris
Jun 5, 2010·RNA Biology·Rachel Nlend NlendDaniel Schümperli
Feb 27, 2013·Nitric Oxide : Biology and Chemistry·Matthew W FosterHarvey E Marshall
Apr 22, 2015·Biomolecules·David PiñeiroEncarna Martinez-Salas
Aug 14, 2013·Biochemical and Biophysical Research Communications·Le Thanh LamGlenn E Morris
Jun 22, 2017·Expert Review of Proteomics·Seyyedmohsen HosseinibarkooieBrunhilde Wirth
Oct 1, 2013·Protein & Cell·Meng-Tzu Weng, Ji Luo
Jun 24, 2010·Journal of Proteome Research·Heidi R FullerGlenn E Morris

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