The snoRNA box C/D motif directs nucleolar targeting and also couples snoRNA synthesis and localization

The EMBO Journal
Dmitry SamarskyE Bertrand

Abstract

Most small nucleolar RNAs (snoRNAs) fall into two families, known as the box C/D and box H/ACA snoRNAs. The various box elements are essential for snoRNA production and for snoRNA-directed modification of rRNA nucleotides. In the case of the box C/D snoRNAs, boxes C and D and an adjoining stem form a vital structure, known as the box C/D motif. Here, we examined expression of natural and artificial box C/D snoRNAs in yeast and mammalian cells, to assess the role of the box C/D motif in snoRNA localization. The results demonstrate that the motif is necessary and sufficient for nucleolar targeting, both in yeast and mammals. Moreover, in mammalian cells, RNA is targeted to coiled bodies as well. Thus, the box C/D motif is the first intranuclear RNA trafficking signal identified for an RNA family. Remarkably, it also couples snoRNA localization with synthesis and, most likely, function. The distribution of snoRNA precursors in mammalian cells suggests that this coupling is provided by a specific protein(s) which binds the box C/D motif during or rapidly after snoRNA transcription. The conserved nature of the box C/D motif indicates that its role in coupling production and localization of snoRNAs is of ancient evolutionary origin.

References

Oct 1, 1992·Experimental Cell Research·K Brasch, R L Ochs
Apr 1, 1992·The Journal of Cell Biology·M Carmo-FonsecaA I Lamond
Jun 1, 1992·Genes & Development·S J BasergaX W Yang
Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J WangT Pederson
Nov 25, 1990·Nucleic Acids Research·J Liu, E S Maxwell
Apr 21, 1995·Cell·E Izaurralde, I W Mattaj
Oct 1, 1995·Genes & Development·W Q Liang, M J Fournier
Jan 1, 1995·Annual Review of Biochemistry·E S Maxwell, M J Fournier
Jul 11, 1995·Nucleic Acids Research·D A SamarskyM J Fournier
Jan 1, 1995·Developmental Genetics·J G GallC Murphy
Dec 22, 1994·Nature·G ZhangM R Green
Nov 25, 1993·Nucleic Acids Research·A G BalakinM J Fournier
May 1, 1993·The Journal of Cell Biology·A G Matera, D C Ward
Sep 1, 1995·Journal of Cellular Biochemistry·I Raska
Nov 1, 1995·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·S A Gerbi
Sep 1, 1996·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·F WeighardtS Riva
Oct 24, 1996·Nature·T Maden
Nov 1, 1996·Current Biology : CB·B A Peculis, S M Mount
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·K T TycowskiJ A Steitz
Jan 1, 1997·Nucleic Acids Research·C Zwieb
Jun 1, 1997·Current Opinion in Cell Biology·D Tollervey, T Kiss
May 30, 1997·Cell·C M Smith, J A Steitz
Jul 1, 1997·Trends in Biochemical Sciences·J P Bachellerie, J Cavaillé
Jul 15, 1997·European Journal of Biochemistry·J D Lewis, E Izaurralde
Jan 31, 1998·The EMBO Journal·B DichtlD Tollervey

❮ Previous
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Citations

May 17, 2011·Cardiovascular Drugs and Therapy·Christopher L Holley, Veli K Topkara
Nov 30, 2002·Biochimie·Jean Pierre BachellerieAlexander Hüttenhofer
Nov 30, 2002·Biochimie·Séverine BoulonCéline Verheggen
Jul 18, 2002·Gene·Irina K GogolevskayaDmitri A Kramerov
Jun 18, 2002·Current Opinion in Cell Biology·Witold Filipowicz, Vanda Pogacić
Jun 6, 2003·Current Opinion in Cell Biology·Susan A GerbiThilo Sascha Lange
Jan 6, 2001·RNA·J VilardellJ R Warner
Apr 1, 2000·RNA·D FilippiniE Caffarelli
Feb 24, 2007·Nature Reviews. Molecular Cell Biology·A Gregory MateraMichael P Terns
Oct 16, 2007·Nature Structural & Molecular Biology·Kum-Loong BoonJean D Beggs
Jul 23, 2002·Molecular Biology of the Cell·Yun Wah LamAngus I Lamond
Nov 17, 1999·Molecular Biology of the Cell·T S LangeS A Gerbi
Jul 9, 1999·Molecular Biology of the Cell·A NarayananM P Terns
Jul 11, 2000·Molecular Biology of the Cell·T S Lange, S A Gerbi
Oct 8, 1998·Molecular Biology of the Cell·T S LangeS A Gerbi
Dec 8, 1998·The EMBO Journal·A HenrasM Caizergues-Ferrer
May 29, 2002·The EMBO Journal·Céline VerheggenEdouard Bertrand
Dec 10, 1998·Nucleic Acids Research·D A Samarsky, M J Fournier
Nov 10, 2000·Nucleic Acids Research·W A SpeckmannM P Terns
Mar 27, 2001·Nucleic Acids Research·L H QuC Yue-Qin
Jul 24, 2002·Nucleic Acids Research·Dan LiangLiang-Hu Qu
Jan 26, 2007·Nucleic Acids Research·Michael P SpillerJean D Beggs
Aug 26, 1998·Genes & Development·E BertrandD R Engelke
May 24, 2003·Cold Spring Harbor Symposia on Quantitative Biology·S A GerbiT S Lange
Mar 27, 2007·Cold Spring Harbor Symposia on Quantitative Biology·T KissM Weber
Mar 25, 2000·Molecular and Cellular Biology·D L Lafontaine, D Tollervey

❮ Previous
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