The solubilization and biological activities of Aspergillus beta-(1 --> 3)-D-glucan

FEMS Immunology and Medical Microbiology
Ken-Ichi IshibashiNaohito Ohno

Abstract

We have recently demonstrated that the cell wall beta-glucan of Candida albicans could be solubilized by sodium hypochlorite, followed by dimethylsulfoxide-extraction (NaClO-DMSO method). In this study, applying this method to Aspergillus spp., we prepared mycelial cell wall beta-glucan and examined its physical properties and immunotoxicological activity. The acetone-dried mycelia of Aspergillus spp. were oxidized by the NaClO-DMSO method. An analysis of (13)C NMR spectra revealed the preparations to be composed of alpha-(1 --> 3) and beta-(1 --> 3)-D-glucan. Also, the proportion of alpha-(1 --> 3) and beta-(1 --> 3)-D-glucan varied. Furthermore, a solubilized Aspergillus beta-glucan (ASBG) was prepared from OX-Asp by urea-autoclave treatment. ASBG showed limulus activity similar to Candida solubilized beta-glucan (CSBG), and there was little difference in the activity of ASBG between various Aspergillus spp. ASBG affected the production of IL-8 by human peripheral blood mononuclear cells (PBMC). ASBG should be useful for analyzing the clinical role of beta-glucan.

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Citations

May 15, 2010·Nihon Ishinkin Gakkai zasshi = Japanese journal of medical mycology·Ken-ichi IshibashiNaohito Ohno
Aug 29, 2013·BioMed Research International·Helioswilton Sales-CamposMárcia Regina Von Zeska Kress
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Mar 19, 2014·Clinical and Experimental Immunology·K IshibashiN Ohno
Jul 6, 2005·Biological & Pharmaceutical Bulletin·Sachiko NamedaNaohito Ohno
Nov 4, 2010·Microbiology and Immunology·Ken-ichi IshibashiNaohito Ohno
Mar 23, 2005·FEMS Immunology and Medical Microbiology·Ken-Ichi IshibashiNaohito Ohno
Jun 14, 2018·Frontiers in Cellular and Infection Microbiology·Jata ShankarPooja Vijayaraghavan
Jun 10, 2009·International Journal of Immunopathology and Pharmacology·K InoueH Takano
May 7, 2021·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Naohito Ohno
Sep 23, 2014·International Journal of Biological Macromolecules·Francini Yumi KagimuraCarlos Ricardo Maneck Malfatti
Jun 13, 2017·International Journal of Biological Macromolecules·Yuina IshimotoNaohito Ohno
Oct 21, 2017·International Journal of Biological Macromolecules·Yuina IshimotoNaohito Ohno
Apr 9, 2005·Revista iberoamericana de micología·Aitor RementeriaJavier Garaizar

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