The soluble isoform of human FcɛRI is an endogenous inhibitor of IgE-mediated mast cell responses.

Allergy
Sherezade Moñino-RomeroZsolt Szépfalusi

Abstract

The soluble isoform of FcɛRI, the high-affinity IgE receptor (sFcεRI), is a protein of the IgE network with poorly defined functions. To define cellular sources and signals that result in the production of human sFcεRI and study its in vivo functions. FcεRI-transfected human cell lines (MelJuso), human monocyte-derived dendritic cells (moDCs), and murine bone marrow-derived mast cells (MC) were stimulated by FcεRI cross-linking and release of sFcεRI was analyzed (ELISA, Western Blot). Lysosomal-associated membrane protein 1 degranulation assays and human basophil activation tests (BATs) were used to study IgE-dependent activation. Recombinant sFcεRI (rsFcεRI) was used to assess its role in murine models of anaphylaxis with WT (wild-type) and IgE-/- (IgE-deficient) mice. Antigen-specific cross-linking of IgE-loaded FcɛRI on MelJuso cells that express the trimeric or tetrameric receptor isoform induced the production of sFcεRI. Using MCs and moDCs, we confirmed that IgE/FcɛRI activation induces sFcɛRI release. We demonstrated that generation of sFcɛRI requires Src phosphorylation and endo/lysosomal acidification. In experimental mouse models, sFcɛRI diminishes the severity of IgE-mediated anaphylaxis. BATs confirmed that, compara...Continue Reading

References

May 1, 1992·The Journal of Experimental Medicine·B WangG Stingl
Aug 4, 1994·Nature·H C OettgenP Leder
Dec 26, 2002·Annual Review of Immunology·Hannah J GouldLyn Smurthwaite
Mar 26, 2003·Clinical and Experimental Dermatology·D von BubnoffT Bieber
Dec 6, 2003·The Journal of Allergy and Clinical Immunology·Calman PrussinThomas B Casale
Mar 9, 2005·The Journal of Allergy and Clinical Immunology·Stephen HolgateColin Reisner
Apr 20, 2005·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·S T HolgateJ Bousquet
Dec 22, 2005·Frontiers in Bioscience : a Journal and Virtual Library·Charles J Malemud
Jun 6, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vadym SulimenkoPavel Dráber
Mar 29, 2007·The Journal of Biological Chemistry·George A LemieuxZena Werb
Apr 18, 2007·Nature Reviews. Immunology·Stefan Kraft, Jean-Pierre Kinet
May 15, 2007·Immunological Reviews·Jean-Pierre Kinet
Oct 24, 2007·The Journal of Experimental Medicine·Mitchell H GraysonMichael J Holtzman
Feb 28, 2008·Nature Reviews. Immunology·Hannah J Gould, Brian J Sutton
Apr 11, 2009·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Juanita MoraLarry L Thomas
May 7, 2011·The Journal of Allergy and Clinical Immunology·Kari C NadeauDale T Umetsu
May 31, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Eva SallmannDieter Maurer
Sep 10, 2011·Journal of Immunological Methods·Willem LexmondEdda Fiebiger
Sep 17, 2011·Immunology Letters·Barbara PlatzerEdda Fiebiger
Aug 29, 2012·Seminars in Immunopathology·Simon P HoganFred D Finkelman
Feb 26, 2013·The New England Journal of Medicine·Marcus MaurerThomas Casale
Jul 3, 2013·International Archives of Allergy and Immunology·Shuba Rajashri IyengarKari Christine Nadeau
Sep 24, 2013·Molecular BioSystems·Joseph S ZhouMenotti Ruvo
Feb 27, 2014·The Journal of Clinical Investigation·Alexandra M GreerJeoung-Sook Shin
Mar 19, 2014·The Journal of Allergy and Clinical Immunology·Michiko K OyoshiPaul J Bryce
Mar 20, 2014·The Journal of Allergy and Clinical Immunology·Alexander EggelTheodore S Jardetzky
Nov 19, 2014·Paediatric Drugs·Amelia LicariGian Luigi Marseglia
Apr 1, 2015·The Journal of Experimental Medicine·Kai-Ting C ShadeRobert M Anthony
Jun 9, 2015·Molecular Immunology·Barbara PlatzerEdda Fiebiger
Oct 21, 2015·The Journal of Allergy and Clinical Immunology·Allen KaplanJames L Zazzali
Oct 29, 2015·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·Eva Serrano-CandelasMarta Ferrer
May 1, 2016·The Journal of Allergy and Clinical Immunology·Fred D FinkelmanRichard Strait
May 20, 2016·Nature Communications·Luke F PenningtonTheodore S Jardetzky
Sep 20, 2016·The Journal of Clinical Investigation·Willem S LexmondEdda Fiebiger
Mar 12, 2017·The Journal of Allergy and Clinical Immunology. in Practice·Yael Gernez, Anna Nowak-Węgrzyn
Jun 19, 2017·The Journal of Allergy and Clinical Immunology·Corey D ClayFred D Finkelman
Jun 21, 2017·International Journal of Molecular Sciences·Leticia de Las Vecillas SánchezMariana C Castells

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Citations

Oct 9, 2019·Allergy·Thomas EiweggerJulia Upton
Jul 25, 2019·Current Opinion in Pediatrics·Zoe C SchmiechenPamela A Frischmeyer-Guerrerio
Jun 7, 2019·F1000Research·Andrea J LukerRebecca K Martin
Nov 7, 2019·Current Opinion in Pediatrics·Zoe C SchmiechenPamela A Frischmeyer-Guerrerio
Feb 7, 2019·Allergy·Sherezade Moñino-RomeroZsolt Szépfalusi
Feb 7, 2020·The Journal of Allergy and Clinical Immunology. in Practice·Sherezade Moñino-RomeroMariana C Castells
Oct 28, 2020·Mucosal Immunology·Evelyn CampbellCathryn R Nagler
Apr 6, 2021·Current Opinion in Immunology·Nicolas Charles
Sep 17, 2021·Clinical Reviews in Allergy & Immunology·Liting HeQianjin Lu

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