The soluble proteome of the Drosophila antenna.

Chemical Senses
Robert R H Anholt, T I Williams

Abstract

The olfactory system of Drosophila melanogaster is one of the best characterized chemosensory systems. Identification of proteins contained in the third antennal segment, the main olfactory organ, has previously relied primarily on immunohistochemistry, and although such studies and in situ hybridization studies are informative, they focus generally on one or few gene products at a time, and quantification is difficult. In addition, purification of native proteins from the antenna is challenging because it is small and encased in a hard cuticle. Here, we describe a simple method for the large-scale detection of soluble proteins from the Drosophila antenna by chromatographic separation of tryptic peptides followed by tandem mass spectrometry with femtomole detection sensitivities. Examination of the identities of these proteins indicates that they originate both from the extracellular perilymph and from the cytoplasm of disrupted cells. We identified enzymes involved with intermediary metabolism, proteins associated with regulation of gene expression, nucleic acid metabolism and protein metabolism, proteins associated with microtubular transport, 8 odorant-binding proteins, protective enzymes associated with antibacterial defens...Continue Reading

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Citations

Jul 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Allison F Carey, John R Carlson
May 25, 2011·Genes, Brain, and Behavior·S SwarupR R H Anholt
Jul 5, 2012·Comparative and Functional Genomics·Jia-Ying ZhuBin Yang
Nov 28, 2013·PloS One·Guido MastrobuoniFrancesca Romana Dani
Feb 24, 2016·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qi ZengScott A Shippy
Oct 31, 2015·Journal of Chemical Ecology·Yunpo ZhaoXuexia Miao
May 21, 2013·Genes, Brain, and Behavior·E B BrownS M Rollmann
Aug 2, 2013·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Fernando MartinEsther Alcorta
Aug 10, 2013·IEEE/ACM Transactions on Computational Biology and Bioinformatics·Qingfeng ChenJianxin Wang
Jan 18, 2015·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Qi ZengScott A Shippy
Jul 20, 2014·Insect Biochemistry and Molecular Biology·Faisal YounusJohn G Oakeshott
Jul 12, 2016·Archives of Insect Biochemistry and Physiology·Ju-Hong ZhangJing-Hui Xi
Sep 30, 2019·Cellular and Molecular Life Sciences : CMLS·Daniel GonzalezLoïc Briand
Aug 26, 2016·G3 : Genes - Genomes - Genetics·John R ShorterRobert R H Anholt
Feb 7, 2020·Journal of Proteomics·Renwen ZhengNemat O Keyhani

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Methods Mentioned

BETA
transgenic

Software Mentioned

Mascot
Sequest

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