The solution structure of type III effector protein AvrPto reveals conformational and dynamic features important for plant pathogenesis

Structure
Jennifer WulfLinda K Nicholson

Abstract

Pseudomonas syringae pv. tomato, the causative agent of bacterial speck disease of tomato, uses a type III secretion system (TTSS) to deliver effector proteins into the host cell. In resistant plants, the bacterial effector protein AvrPto physically interacts with the host Pto kinase and elicits antibacterial defense responses. In susceptible plants, which lack the Pto kinase, AvrPto acts as a virulence factor to promote bacterial growth. The solution structure of AvrPto reveals a functional core consisting of a three-helix bundle motif flanked by disordered N- and C-terminal tails. Residues required for Pto binding lie in a 19 residue Omega loop. Modeling suggests a hydrophobic patch involving the activation loop of Pto forms a contact surface with the AvrPto Omega loop and that helix packing mediates interactions between AvrPto and putative virulence targets Api2 and Api3. The AvrPto structure has a low stability that may facilitate chaperone-independent secretion by the TTSS.

References

Oct 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J S Cha, D A Cooksey
Nov 1, 1989·Analytical Biochemistry·S C Gill, P H von Hippel
Nov 14, 1986·Science·J F Leszczynski, G D Rose
Nov 1, 1995·Journal of Biomolecular NMR·F DelaglioA Bax
Nov 1, 1995·Proteins·T MadejS H Bryant
Dec 1, 1996·Journal of Biomolecular NMR·R A LaskowskiJ M Thornton
Oct 27, 1999·Proceedings of the National Academy of Sciences of the United States of America·D M AndersonO Schneewind
Nov 5, 1999·Journal of Molecular Biology·P E Wright, H J Dyson
May 5, 2000·Molecular Plant-microbe Interactions : MPMI·J H ChangR W Michelmore
Jun 1, 2000·Molecular Plant-microbe Interactions : MPMI·L ShanX Tang
Aug 2, 2000·Proceedings of the National Academy of Sciences of the United States of America·A J Bogdanove, G B Martin
Jan 3, 2001·Nature Structural Biology·M WürteleA Wittinghofer
Jan 3, 2001·Molecular Microbiology·S A LloydH Wolf-Watz
Apr 20, 2001·Molecular Plant-microbe Interactions : MPMI·J H ChangR W Michelmore
Jul 27, 2001·Nature Structural Biology·K W Plaxco, M Gross
Aug 17, 2001·Nature·C E Stebbins, J E Galán
Nov 8, 2001·Journal of Molecular Biology·C WangL K Nicholson
Feb 14, 2002·Journal of the American Chemical Society·Amr Fahmy, Gerhard Wagner
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Derrick E FoutsAlan Collmer
Sep 13, 2002·Journal of the American Chemical Society·Qing-Hong DaiA Joshua Wand
Sep 20, 2002·Journal of Biomolecular NMR·Jennifer WulfLinda K Nicholson
Feb 7, 2003·The Journal of Biological Chemistry·Frank S CordesSusan M Lea
Mar 7, 2003·FEMS Microbiology Letters·Mario F Feldman, Guy R Cornelis
Jun 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·Paula HauckSheng Yang He
Jul 23, 2003·Current Opinion in Plant Biology·Daniela Büttner, Ulla Bonas
Aug 21, 2003·Proceedings of the National Academy of Sciences of the United States of America·C Robin BuellAlan Collmer
Aug 30, 2003·Science·Feng ShaoRoger W Innes
Sep 25, 2003·Annual Review of Plant Biology·Gregory B MartinGuido Sessa
Sep 25, 2003·Nature Reviews. Molecular Cell Biology·C Erec Stebbins, Jorge E Galán
Oct 7, 2003·Annual Review of Phytopathology·Kerry F Pedley, Gregory B Martin

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Citations

Aug 29, 2006·Nature Reviews. Molecular Cell Biology·Robert B AbramovitchGregory B Martin
May 9, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jennifer E DawsonLinda K Nicholson
Mar 15, 2006·Molecular Plant-microbe Interactions : MPMI·Jorge L BadelAlan Collmer
Jun 7, 2006·Annual Review of Microbiology·Sarah R GrantJeffery L Dangl
Feb 22, 2012·Trends in Microbiology·Magdalen LindebergAlan Collmer
Feb 14, 2009·Cellular Microbiology·Anastasia D GaziMichael Kokkinidis
Dec 22, 2005·The Plant Journal : for Cell and Molecular Biology·Jeroen S de VriesJohn P Rathjen
Jun 26, 2008·Protein Science : a Publication of the Protein Society·Jennifer E Dawson, Linda K Nicholson
Sep 4, 2007·The Plant Journal : for Cell and Molecular Biology·Fangming XiaoGregory B Martin
May 23, 2006·Current Opinion in Plant Biology·Darrell DesveauxJeffery L Dangl
Nov 3, 2005·Current Opinion in Structural Biology·Steven JohnsonSusan M Lea
Mar 13, 2008·Current Biology : CB·Cyril Zipfel, John P Rathjen
Jul 10, 2004·Structure·David A Horita
Mar 3, 2010·Molecular Plant-microbe Interactions : MPMI·Suparat KunkeawGitta Coaker
Apr 5, 2014·Chemical Reviews·Macarena Marín, Thomas Ott
Oct 9, 2013·Nature Reviews. Microbiology·Lennart WirthmuellerMark J Banfield
Jun 3, 2021·International Journal of Molecular Sciences·Anastasia D GaziVasiliki E Fadouloglou

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