The solution structure of yeast tRNAPhe as studied by nuclear Overhauser effects in NMR

Journal of Biomolecular Structure & Dynamics
C W HilbersJ A Walters

Abstract

Recently, the imino proton spectrum of yeast tRNAPhe has been assigned by means of the application of the nuclear Overhauser effect (NOE). In the present paper it will be shown that even for tRNA (MW 28000) connectivities between the imino proton spins can be observed using two-dimensional NOE spectroscopy. In this way the imino proton resonances of the D-stem region are assigned. The results are discussed in relation to those obtained by the classical one-dimensional nuclear Overhauser effect. It turns out that in 2D-NOE experiments connectivities from overlapping resonances can be observed which cannot be determined by one-dimensional Overhauser experiments. Moreover, the total assignment of the imino proton spectrum of yeast tRNAPhe is used to relate the three-dimensional crystal structure of the tRNA to its solution structure. It is shown that the principle elements of the X-ray structure, i.e. the hydrogen bonding network and the stacking of the stems upon one another, are also found in solution. This is true for the presence as well as for the absence of magnesium ions. However, in absence of magnesium ions the tRNA structure appears to differ in details from that in the presence of magnesium ions. Finally, the influence ...Continue Reading

References

Jan 1, 1976·Annual Review of Biochemistry·A Rich, U L RajBhandary
Jul 1, 1977·Biopolymers·E R HawkinsW L Mattice
May 1, 1978·Nucleic Acids Research·C Urbanke, G Maass
Aug 25, 1978·Journal of Molecular Biology·J L SussmanS H Kim
Sep 25, 1978·Journal of Molecular Biology·B HingertyA Jack
Mar 8, 1979·Nature·R W SchevitzJ L Sussman
Jul 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·M Bina-SteinR G Shulman
Jan 1, 1973·Journal of Molecular Biology·J Eisinger, P F Spahr
Mar 5, 1973·Journal of Molecular Biology·P K Webb, J R Fresco
Nov 5, 1974·Journal of Molecular Biology·O C UhlenbeckJ R Fresco
Jul 30, 1982·Biochimica Et Biophysica Acta·S CorbinB Pullman
Apr 11, 1983·Nucleic Acids Research·J S Tropp, A G Redfield
Oct 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·V SánchezJ Tropp
Dec 25, 1980·Nature·D MorasR Giegé
Aug 5, 1980·Biochemistry·D Labuda, D Pörschke

❮ Previous
Next ❯

Citations

Aug 1, 1986·Biophysical Chemistry·F Jelen, E Palecek
Jan 1, 1986·Bio Systems·R CedergrenB Larue
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A DelbarreB P Roques
May 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·M W FriederichP J Hagerman
Feb 1, 1985·Journal of Biomolecular Structure & Dynamics·J L LeroyM Guérón
Jun 1, 1986·Journal of Biomolecular Structure & Dynamics·R OsterbergR Bauer
Jul 11, 1989·Biochemistry·K B HallA G Redfield
Dec 29, 1992·Biochemistry·B S Choi, A G Redfield
Dec 1, 1988·European Journal of Biochemistry·F J Van de Ven, C W Hilbers
Feb 17, 1986·European Journal of Biochemistry·E I Hyde
Nov 11, 1991·Quarterly Reviews of Biophysics·G Varani, I Tinoco
Aug 1, 1987·Quarterly Reviews of Biophysics·D J PatelD Hare
Oct 1, 1989·Journal of Biomolecular Structure & Dynamics·G StrikerM C Vega-Martin
Oct 30, 2007·FEBS Letters·Elisabetta Viani Puglisi, Joseph D Puglisi
Jan 1, 1988·Critical Reviews in Clinical Laboratory Sciences·J A Shafer, D L Higgins
Feb 24, 2021·Critical Reviews in Biochemistry and Molecular Biology·Yasemin YoluçStefanie Kellner
Apr 8, 1986·Biochemistry·B S Choi, A G Redfield
Aug 5, 2009·Methods : a Companion to Methods in Enzymology·John K Frederiksen, Joseph A Piccirilli

❮ Previous
Next ❯

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