PMID: 8609161Apr 1, 1996Paper

The sorting sequence of the peroxisomal integral membrane protein PMP47 is contained within a short hydrophilic loop

The Journal of Cell Biology
J M DyerJ M Goodman

Abstract

No targeting sequence for peroxisomal integral membrane proteins has yet been identified. We have previously shown that a region of 67 amino acids is necessary to target Pmp47, a protein that spans the membrane six times, to peroxisomes. This region comprises two membrane spans and the intervening loop. We now demonstrate that the 20 amino acid loop, which is predicted to face the matrix, is both necessary and sufficient for peroxisomal targeting. Sufficiency was demonstrated with both chloramphenicol acetyltransferase and green fluorescent protein as carriers. There is a cluster of basic amino acids in the middle of the loop that we predict protrudes from the membrane surface into the matrix by a flanking stem structure. We show that the targeting signal is composed of this basic cluster and a block of amino acids immediately down-stream from it.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1991·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·A G Bodnar, R A Rachubinski
May 3, 1991·Cell·P BassfordB Wickner
Feb 3, 1989·Science·W F DeGradoJ D Lear
May 1, 1989·The Journal of Cell Biology·S J GouldS Subramani
Nov 14, 1986·Science·J F Leszczynski, G D Rose
Jan 1, 1985·Annual Review of Cell Biology·P B Lazarow, Y Fujiki
May 5, 1982·Journal of Molecular Biology·J Kyte, R F Doolittle
Jun 1, 1995·Molecular Biology of the Cell·P A WaltonS Subramani
Apr 1, 1995·The Journal of Cell Biology·P A MarshallJ M Goodman
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·J R GloverR A Rachubinski
Dec 1, 1994·The Journal of Cell Biology·J A McNew, J M Goodman
Aug 30, 1994·Biochimica Et Biophysica Acta·E Schwarz, W Neupert
Jun 6, 1994·FEBS Letters·B JungnickelE Hartmann
Nov 1, 1993·Trends in Biochemical Sciences·X H Feng, S D Kung
Jan 1, 1993·Critical Reviews in Biochemistry and Molecular Biology·J Kuan, M H Saier
Feb 1, 1993·Molecular Biology of the Cell·J A McNewJ M Goodman

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Citations

Jan 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·H R Waterham, J M Cregg
Dec 14, 2011·Journal of Industrial Microbiology & Biotechnology·Juan-Francisco MartínCarlos García-Estrada
Jan 22, 2004·Biochemical and Biophysical Research Communications·Joanne E Hunt, Richard N Trelease
May 30, 2001·Molecular and Biochemical Parasitology·M ParsonsP Kessler
Jun 8, 2000·FEMS Microbiology Reviews·R J BaerendsM Veenhuis
May 30, 2001·Trends in Plant Science·R T MullenR N Trelease
May 2, 2001·Nature Reviews. Molecular Cell Biology·V I Titorenko, R A Rachubinski
Apr 1, 1997·Nature Genetics·S Subramani
Oct 1, 1996·The Journal of Cell Biology·P A MarshallJ M Goodman
May 11, 2004·Molecular Biology of the Cell·Hanspeter RottensteinerRalf Erdmann
Jun 8, 2001·Molecular and Cellular Biology·M FransenP P Van Veldhoven
Nov 1, 2001·Annual Review of Cell and Developmental Biology·P E Purdue, P B Lazarow
Oct 7, 2003·Annual Review of Genomics and Human Genetics·Sabine WellerDavid Valle
Nov 30, 2010·Therapeutic Delivery·Mohanad MossalamCarol S Lim
Jul 17, 2001·Biochemical and Biophysical Research Communications·M Biermanns, J Gärtner
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·F E WeberH Hauser
Feb 27, 2008·DNA Sequence : the Journal of DNA Sequencing and Mapping·Lingyong LiDeyue Yu
Jun 7, 2006·Molecular Membrane Biology·Cédric YernauxPaul A M Michels
Jun 1, 2006·The Journal of Cell Biology·Derk BinnsJoel M Goodman
May 23, 1998·The Journal of Cell Biology·M PassreiterW W Just
Jul 29, 1998·The Journal of Cell Biology·E H HettemaI Braakman
Mar 10, 2004·The Journal of Cell Biology·Yi FangStephen J Gould
Apr 13, 2000·FEBS Letters·B PauseW W Just
Jun 13, 2001·The Journal of Cell Biology·J M JonesS J Gould
Jan 1, 1996·Critical Reviews in Biotechnology·A EndrizziJ M Belin

❮ Previous
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