The SOS Response Master Regulator LexA Regulates the Gene Transfer Agent of Rhodobacter capsulatus and Represses Transcription of the Signal Transduction Protein CckA

Journal of Bacteriology
Kevin S KuchinskiJ Thomas Beatty

Abstract

The gene transfer agent of Rhodobacter capsulatus (RcGTA) is a genetic exchange element that combines central aspects of bacteriophage-mediated transduction and natural transformation. RcGTA particles resemble a small double-stranded DNA bacteriophage, package random ∼4-kb fragments of the producing cell genome, and are released from a subpopulation (<1%) of cells in a stationary-phase culture. RcGTA particles deliver this DNA to surrounding R. capsulatus cells, and the DNA is integrated into the recipient genome though a process that requires homologs of natural transformation genes and RecA-mediated homologous recombination. Here, we report the identification of the LexA repressor, the master regulator of the SOS response in many bacteria, as a regulator of RcGTA activity. Deletion of the lexA gene resulted in the abolition of detectable RcGTA production and an ∼10-fold reduction in recipient capability. A search for SOS box sequences in the R. capsulatus genome sequence identified a number of putative binding sites located 5' of typical SOS response coding sequences and also 5' of the RcGTA regulatory gene cckA, which encodes a hybrid histidine kinase homolog. Expression of cckA was increased >5-fold in the lexA mutant, and ...Continue Reading

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Citations

Jun 28, 2016·Molecular Biology and Evolution·Alexander P HynesAndrew S Lang
Oct 6, 2016·Annals of the New York Academy of Sciences·Michael R Gillings
Apr 8, 2018·Applied and Environmental Microbiology·Alexander B WestbyeJ Thomas Beatty
Dec 19, 2017·Virus Evolution·Migun ShakyaOlga Zhaxybayeva
Oct 30, 2019·Journal of Bacteriology·Purvikalyan PallegarAndrew S Lang
Mar 10, 2020·Metallomics : Integrated Biometal Science·Nur SelamogluFevzi Daldal
Aug 9, 2017·Annual Review of Virology·Andrew S LangJ Thomas Beatty
Sep 26, 2020·Biochemical and Biophysical Research Communications·James A Shapiro

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