The source of Mycobacterium tuberculosis-specific IFN-γ production in peripheral blood mononuclear cells of TB patients

International Immunopharmacology
Feng WangZiyong Sun

Abstract

Mycobacterium tuberculosis (Mtb)-specific IFN-γ secretion plays important roles in anti-tuberculosis (TB) immunity. Mtb-specific IFN-γ response can be induced in HIV/TB co-infected patients with a low CD4 lymphocyte count; this suggests that the source of Mtb-specific IFN-γ production is not limited in CD4(+) T lymphocytes. Currently, the major sources of Mtb-specific IFN-γ production and the function and phenotype of Mtb-specific IFN-γ-producing cells still remain unclear. Thirty-nine participants (24 active TB patients, 10 HIV/TB co-infected patients, and 5 healthy volunteers) were recruited according to conventional tests and Mtb-specific IFN-γ ELISPOT assay. Multicolor flow cytometry was used to investigate the production of intracellular IFN-γ in peripheral blood mononuclear cells (PBMCs) after Mtb-specific antigen stimulation. Our results showed that CD4(+), CD8(+) T cells and NK cells are all major sources of Mtb-specific IFN-γ production in PBMCs of TB patients. Moreover, CD8(+) T cells are the highest number of Mtb-specific IFN-γ-producing cells in HIV/TB co-infected patients. Although the activity of NK cells is significantly reduced in TB patients when compared with healthy controls, Mtb-specific antigen stimulation ...Continue Reading

References

Jul 12, 2001·Infection and Immunity·B RajuD B Tse
Aug 28, 2003·Nature Immunology·Robert A Seder, Rafi Ahmed
Dec 9, 2003·Journal of Immunological Methods·Annika C KarlssonElizabeth Sinclair
Mar 23, 2004·Annual Review of Immunology·Federica SallustoAntonio Lanzavecchia
Nov 30, 2004·The Lancet Infectious Diseases·Madhukar PaiJohn M Colford
Jul 11, 2006·Immunological Reviews·Brigitta StockingerGeorge Kassiotis
Dec 13, 2006·American Journal of Respiratory and Critical Care Medicine·Molebogeng Xheeda RangakaRobert J Wilkinson
Jan 16, 2007·American Journal of Respiratory and Critical Care Medicine·Annie F LuetkemeyerDiane V Havlir
Jan 24, 2009·Immunological Reviews·Andrea M Cooper, Shabaana A Khader
May 30, 2009·PloS One·Basirudeen Syed Ahamed KabeerAlamelu Raja
Apr 9, 2011·Nature Reviews. Immunology·Gerhard WalzlAlimuddin Zumla
Aug 20, 2011·PloS One·Zhudong LiuJianghua Sun
Apr 12, 2012·World Journal of Gastroenterology : WJG·Jian-Hua ChenZheng-Hao Tang
Dec 11, 2013·PloS One·Melissa R NyendakUNKNOWN Tuberculosis Research Unit and the Tuberculosis Trials Consortium
Sep 6, 2014·Journal of Clinical Immunology·Zitao LiChangyou Wu
Mar 27, 2015·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Rebecca Axelsson-RobertsonMarkus Maeurer
Jul 21, 2015·Nature Immunology·R Keith ReevesDan H Barouch

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Citations

Feb 22, 2017·Journal of Clinical Laboratory Analysis·Jun-Chi XuPing Xu
Apr 11, 2017·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Munyemana Jean BoscoFeng Wang
Oct 19, 2016·The Pediatric Infectious Disease Journal·Laura PöyhönenMatti Korppi
Feb 13, 2020·Journal of Cellular and Molecular Medicine·Xing-Hao YiZheng-Jun Yi

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