The Sp1 transcription factor regulates cell type-specific transcription of MUC1

DNA and Cell Biology
J Morris, J Taylor-Papadimitriou

Abstract

Because the MUC1 mucin is highly expressed in breast and other carcinomas, interest is focused on the MUC1 promoter, particularly in the context of the delivery of genes to carcinomas. Earlier in vitro studies showed that the region between -152 and -66 of the MUC1 promoter is required for transcriptional activity in MUC1-expressing cells. Experiments reported here showed that sequences -119/-62 within this region are able to modulate transcription of the heterologous constitutively active herpes simplex virus thymidine kinase promoter in a pattern consistent with MUC1 expression. Band-shift experiments showed that although several factors (including Sp1 and Sp3) bind to these sequences, the element important in directing this MUC1 pattern of expression was an Sp1 GC box at -97. The data also show that the positioning or phase of the GC box was crucial for directing expression. The importance of the Sp1 transcription factor was confirmed by demonstrating that overexpression of Sp1 in MUC1-nonexpressing cells increased, not only the expression of a reporter gene driven by the 1.4-kb MUC1 promoter, but also the expression of MUC1 from the endogenous gene. Together, these data define an important role for Sp1 in the cell type-spec...Continue Reading

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Citations

Apr 23, 2002·Journal of Cellular Biochemistry·Yasuhiro MaruyamaBeatrice Y J T Yue
Jun 19, 2001·DNA and Cell Biology·A G ScibettaJ Taylor-Papadimitriou
Jun 30, 2007·American Journal of Respiratory Cell and Molecular Biology·Ippei KuwaharaK Chul Kim
Jan 9, 2004·Reproductive Biology and Endocrinology : RB&E·Melissa BraymanDaniel D Carson
Apr 6, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·A W HorneJ O White
May 16, 2002·Chest·George D LeikaufLeigh G Simpson
Jan 15, 2009·Biochemical and Biophysical Research Communications·Yuji MikamiHiroshi Katsuki
Sep 9, 2015·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Janine RegneriManfred Schartl
Feb 21, 2006·Biochimica Et Biophysica Acta·Mahefatiana AndrianifahananaSurinder K Batra
Feb 20, 2016·International Journal of Biological Macromolecules·Mahadevappa HemshekharKesturu S Girish
Apr 26, 2005·American Journal of Physiology. Lung Cellular and Molecular Physiology·Ippei KuwaharaK Chul Kim
Jul 1, 2008·Expert Review of Endocrinology & Metabolism·Daniel D CarsonPeng Wang

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