The species Mycobacterium africanum in the light of new molecular markers

Journal of Clinical Microbiology
Stefan NiemannSabine Rüsch-Gerdes

Abstract

The findings of recent studies addressing the molecular characteristics of Mycobacterium tuberculosis complex isolates have initiated a discussion on the classification of M. africanum, especially of those isolates originating from East Africa (cluster F, subtype II) and displaying phenotypic and biochemical characteristics more similar to those of M. tuberculosis. To further address this question, we analyzed a representative collection of 63 M. tuberculosis complex strains comprising 30 M. africanum subtype I strains, 20 M. africanum subtype II strains, 10 randomly chosen M. tuberculosis isolates, and type strains of M. tuberculosis, M. bovis, and M. africanum for the following biochemical and molecular characteristics: single-nucleotide polymorphisms (SNPs) in gyrB and narGHJI and the presence or absence of RD1, RD9, and RD12. For all molecular markers analyzed, subtype II strains were identical to the M. tuberculosis strains tested. In contrast, the subtype I strains as well as the M. africanum type strain showed unique combinations of SNPs in gyrB and genomic deletions (the absence of RD9 and the presence of RD12), which proves their independence from M. tuberculosis and M. bovis. Accordingly, all subtype I strains display...Continue Reading

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Citations

Oct 23, 2008·Human Molecular Genetics·Thorsten ThyeChristian G Meyer
Nov 8, 2005·Journal of Clinical Microbiology·Kristin KremerDick van Soolingen
Jan 5, 2006·Journal of Clinical Microbiology·Simeon CadmusStephen V Gordon
Jun 27, 2008·BMC Microbiology·Susanne HomolkaStefan Niemann
Mar 31, 2010·BMC Infectious Diseases·Sidra E Gonçalves VasconcellosJohn L Ho
Nov 8, 2012·BMC Clinical Pathology·James GafiritaBenon B Asiimwe
Jul 12, 2008·PLoS Neglected Tropical Diseases·Zoheira DjelouadjiMichel Drancourt
May 3, 2011·PloS One·Ramona GroenheitGunilla Källenius
Feb 2, 2010·Emerging Infectious Diseases·Timothy BrownFrancis Drobniewski
Jun 12, 2010·International Journal of Medical Microbiology : IJMM·Susanne HomolkaStefan Niemann
May 5, 2009·Enfermedades infecciosas y microbiología clínica·Laura Herrera-LeónMaría Soledad Jiménez Pajares
Dec 23, 2008·Enfermedades infecciosas y microbiología clínica·Iván Pérez-de PedroMaría Soledad Jiménez
Oct 26, 2005·Journal of Theoretical Biology·Noel H SmithJohn Maynard Smith
May 23, 2014·Veterinary Medicine International·Sydney MalamaJacques Godfroid
Dec 17, 2009·Scandinavian Journal of Infectious Diseases·Marianne Kirstine KjeldsenVibeke Østergaard Thomsen
Apr 6, 2019·Open Forum Infectious Diseases·Arpita ChakravartiChristian Lavallée
Jan 14, 2017·Microbiology Spectrum·Jaime Esteban, Maria-Carmen Muñoz-Egea
Aug 10, 2021·Frontiers in Public Health·Prince AsareDorothy Yeboah-Manu

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