PMID: 7543410Jul 15, 1995Paper

The species-specific differences in the cAMP regulation of the tissue-type plasminogen activator gene between rat, mouse and human is caused by a one-nucleotide substitution in the cAMP-responsive element of the promoters

European Journal of Biochemistry
M HolmbergT Ny

Abstract

In rat ovarian cells tissue-type plasminogen activator (tPA) is induced by gonadotropins, by a cAMP-dependent pathway and the induction correlates with the time of follicle rupture in vivo. However, in mice, gonadotropins induce the related but distinct protease urokinase-type plasminogen activator (uPA). Comparison of rat, mouse and human tPA genes reveal that there is a species-specific difference in the promoter that could explain the difference in regulation of the tPA gene between these species. At the position where the rat promoter contains a consensus cAMP-responsive element (CRE), the mouse and human counterparts contains a CRE variant with a one-nucleotide substitution. Transient transfection experiments of rat glial and granulosa cells demonstrated that reporter constructs driven by rat but not mouse or human tPA promoters were efficiently induced by the cAMP-inducing agents forskolin or follicle-stimulating hormone. Following the conversion of the mouse and human CRE-like sequences to rat consensus CRE these promoters became cAMP responsive. In contrast the rat promoter, following conversion of the consensus CRE to the corresponding mouse and human CRE-like sequence, lost the ability to efficiently respond to cAMP. ...Continue Reading

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Citations

Aug 8, 2002·Kidney International·Wolfgang EberhardtJosef Pfeilschifter
Sep 17, 1996·Proceedings of the National Academy of Sciences of the United States of America·S MajumdarH P Elsholtz
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·N W SeedsS P Haffke
Dec 3, 2008·Brain Research·George A RoggeMichael J Kuhar
Dec 16, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·K KarikóF A Welsh
May 24, 2014·Gene Regulation and Systems Biology·Anna Tjärnlund-WolfChristina Jern
Jul 26, 2002·Journal of Inherited Metabolic Disease·D N Cooper

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