PMID: 7090023Mar 1, 1982Paper

The specific binding of estradiol and estrone and the subsequent distribution of estrogen-receptor complexes within MCF-7 human breast cancer cells

Steroids
J MacIndoeL A Etre

Abstract

We have examined the specific binding of estradiol (E2) and estrone (E1) within MCF-7 cells using both cytosol and whole cell suspension binding assay techniques. The results of these studies have revealed that each of these estrogens binds the high but different affinity to the same single class of cytosol receptor (ER). The incubation of intact cells with E2 at 37 degrees resulted in the rapid formation, nuclear binding, and nuclear processing of E2-ER complexes. Reduction of incubation temperature to 15 degrees C and 4 degrees C resulted in slowed but continued E2-ER formation and nuclear binding, and in a marked inhibition of nuclear receptor processing. The incubation of intact cells with E1 at 37 degrees also was associated with the formation, nuclear binding, and nuclear processing of estrogen-ER complexes. Interestingly, however, E2 rather the E1 represented the major species of specifically bound estrogen under these conditions. This observation suggests that the estrogenic action of E1 in MCF-7 cells may be mediated largely by the intracellular formation of E2. This phenomenon provides a likely explanation of the unexpected potency of E1 in this system previously observed by other workers. Furthermore, our results sug...Continue Reading

References

Aug 1, 1979·The Journal of Clinical Endocrinology and Metabolism·J MacIndoe
Jan 1, 1971·Analytical Biochemistry·R T Hinegardner

Citations

Oct 1, 1990·Breast Cancer Research and Treatment·J MacIndoeG Woods
Jan 1, 1985·Breast Cancer Research and Treatment·P M LohW D Peterson
Dec 1, 1985·Journal of Steroid Biochemistry·R C BonneyV H James
Jul 1, 1989·Molecular and Cellular Endocrinology·R W BrueggemeierJ M Stevens
Sep 27, 2001·Antisense & Nucleic Acid Drug Development·A H TaylorF Al-Azzawi
Apr 22, 1993·International Journal of Cancer. Journal International Du Cancer·S J SantnerR J Santen
Jan 1, 1988·Pharmacology & Therapeutics·A A Sandberg, R Y Kirdani
Aug 1, 1985·European Journal of Cancer & Clinical Oncology·N Olea-SerranoJ C Heuson
Jan 15, 1991·Biochemical and Biophysical Research Communications·G E Swaneck, J Fishman
Jan 1, 1983·Journal of Cellular Biochemistry·W D BloomerS J Adelstein
Apr 5, 2003·Environmental Health Perspectives·Jody L RalphColleen C Nelson

Related Concepts

Mammary Neoplasms, Human
Cell Nucleus
Cold Temperature
Cytoplasmic Matrix
Estradiol, (16 alpha,17 beta)-Isomer
Unigen
Estrogen Nuclear Receptor
Drug or Chemical Tissue Distribution

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