PMID: 11325424Apr 28, 2001Paper

The specific hydrolysis of HIV-1 TAR RNA element with the anti-TAR hammerhead ribozyme: structural and functional implications

International Journal of Biological Macromolecules
E WyszkoJ Barciszewski

Abstract

The main transcriptional regulator of the human immunodeficiency virus is the Tat protein, which recognises and binds to a fragment RNA at the 5' end of viral mRNA, named transactivation response element (TAR) RNA. Extensive mutagenesis studies have shown that a region of TAR RNA important for Tat binding involves a set of nucleotides surrounding a characteristic UCU nucleotide bulge. The specific Tat-TAR complex formation enhances the rate of transcription elongation but inhibition of that interaction prevents the human immunodeficiency virus type 1 (HIV-1) replication. If so, a possibility of virus inactivation would be a site specific degradation of the TAR RNA element. To break down and inactivate TAR RNA, we designated the anti-hammerhead (HH) ribozyme to cleave nucleosides within the bulge. We showed for the first time the new type of the AUC hammerhead ribozyme, which hydrolyses specifically the TAR RNA element at C8 nucleotide in the bulge (C24 in the standard TAR RNA numbering). The cleavage reaction has broad magnesium requirements. Mn and particularly Ca are less efficient. Argininamide interferes with the cleavage of TAR RNA induced by the ribozyme. These results have two implications; (i) structural, where the HIV-...Continue Reading

References

Aug 1, 1977·Nucleic Acids Research·S R HolbrookS H Kim
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J D PuglisiJ R Williamson
Jul 1, 1993·Trends in Biochemical Sciences·M J Gait, J Karn
Jan 1, 1996·Nature Structural Biology·W I Sundquist
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·A C JamiesonS H Kim
Jul 15, 1997·Nucleic Acids Research·R H Symons
Jan 22, 1998·Proceedings of the National Academy of Sciences of the United States of America·W B LottP H von Hippel
Jun 6, 1998·Nucleic Acids Research·A S BrodskyJ R Williamson
Jul 17, 1998·Biochemistry·H SugaJ W Szostak
Aug 13, 1998·RNA·T K Stage-Zimmermann, O C Uhlenbeck
Sep 2, 1998·Trends in Genetics : TIG·J J Rossi
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·D Thirumalai
Sep 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·M Wu, I Tinoco
Nov 10, 1998·Trends in Biotechnology·B BramlageF Eckstein
Nov 10, 1998·Nature Structural Biology·S RowsellS E Phillips
Nov 25, 1998·Nucleic Acids Research·N K VaishF Eckstein
Dec 8, 1998·Biochemistry·J M Bevilacqua, P C Bevilacqua
Dec 10, 1998·Nucleic Acids Research·M SzymanskiV A Erdmann
Jan 26, 1999·Antisense & Nucleic Acid Drug Development·R KirchnerM Sprinzl
Feb 3, 1999·Nucleic Acids Research·Y TanakaY Kyogoku
Mar 2, 1999·FEBS Letters·V A PetyukV V Vlassov

❮ Previous
Next ❯

Citations

Jun 23, 2004·Biochemical and Biophysical Research Communications·Hong ZhaoLong Jiang
Nov 26, 2003·Biochemical and Biophysical Research Communications·Hong ZhaoLong Jiang
Apr 16, 2016·Scientific Reports·Agnieszka BelterJan Barciszewski
Apr 6, 2004·FEBS Letters·Hong ZhaoLong Jiang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.