PMID: 8580971Jan 1, 1996Paper

The spinach AtpC and AtpD genes contain elements for light-regulated, plastid-dependent and organ-specific expression in the vicinity of the transcription start sites

The Plant Journal : for Cell and Molecular Biology
C BolleR Oelmüller

Abstract

Run-on assays with isolated nuclei demonstrate that the transcription rates of AtpC and AtpD (gene products: the CF1 subunits gamma and delta of the chloroplast ATP synthase) are comparable in spinach seedlings. However, chimeric GUS gene fusions with 5'-flanking regions of the AtpC gene direct an approximately 10-fold lower GUS level in transgenic tobacco compared with equivalent fragments from the AtpD gene. Both promoters contain sequences in the vicinity of the respective TATA boxes, which are sufficient to direct light-regulated, plastid-dependent and organ-specific expression of the GUS gene. In contrast, the upstream regions of both promoters differ the higher GUS level directed by the AtpD promoter is caused by enhancer-like elements located upstream of the region involved in the regulated expression, while nucleotides upstream of -73 in the AtpC promoter contribute relatively little to the promoter activity. 5'-Deletion analyses and site-directed mutagenesis studies indicated that the -73/-48 bp AtpC region contains cis-elements crucial for this regulated expression. If five nucleotides within this region (-59/-55) are exchanged, the GUS gene is constitutively expressed and the activity in etiolated seedlings, in seedl...Continue Reading

Citations

Oct 19, 2005·Planta·Christoph F Beck
Apr 25, 2012·Plant Molecular Biology·Katrin PetersHans-Peter Braun
Jan 3, 2001·The Plant Journal : for Cell and Molecular Biology·G VintiE López-Juez
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Gerardo Arguello-Astorga, Luis Herrera-Estrella
May 4, 2006·Annual Review of Plant Biology·Ajit NottJoanne Chory
Feb 5, 2004·Genome Biology·Karen E ThumGloria M Coruzzi
Feb 12, 2013·Annual Review of Plant Biology·Wei ChiLixin Zhang

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