The spindle position checkpoint requires positional feedback from cytoplasmic microtubules.

Current Biology : CB
Jeffrey K MooreJohn A Cooper

Abstract

The objective of mitosis is to provide a copy of the genome to each progeny of a cell division. This requires the separation of duplicate chromatids by the spindle apparatus and the delivery of one set of chromosomes to each of the daughter cells. In budding yeast, the fidelity of chromosome delivery depends on the spindle position checkpoint, which prolongs mitosis until one end of the anaphase spindle arrives in the bud. Here we tested the hypothesis that the activity of the spindle position checkpoint depends on persistent interactions between cytoplasmic microtubules and the mother-bud neck, the future site of cytokinesis. We used laser ablation to disrupt microtubule interactions with the bud neck, and we found that loss of microtubules from the neck leads to mitotic exit in a majority of checkpoint-activated cells. Our findings suggest that cytoplasmic microtubules are used to monitor the location of the spindle in the dividing cell and, in the event of positioning errors, relay a signal to inhibit mitotic exit until the spindle is appropriately positioned.

References

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Citations

Dec 15, 2010·Cell Division·Ayse K CaydasiGislene Pereira
Mar 13, 2014·The Journal of Cell Biology·Rania S RizkMohan L Gupta
Nov 3, 2010·The Journal of Cell Biology·Jeffrey K MooreJohn A Cooper
Dec 15, 2010·The Journal of Cell Biology·Marco GeymonatSteven G Sedgwick
Sep 22, 2010·The Journal of Cell Biology·Ayse Koca CaydasiGislene Pereira
Aug 28, 2015·Molecular Biology of the Cell·Sabyasachi SutradharKaustuv Sanyal
Apr 19, 2012·Experimental Cell Research·Ayse Koca Caydasi, Gislene Pereira
May 1, 2012·Experimental Cell Research·Steven M MarkusWei-Lih Lee
May 12, 2016·ELife·Jill Elaine FalkAngelika Amon
Sep 7, 2018·Journal of Cell Science·Zane J BergmanGeorjana Barnes
Apr 13, 2012·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jun WuTanmay P Lele

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Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.

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