Abstract
The relative paucity of data about the development of the stem cell pool present in the spleen prompted this study. During in vitro cultures of B-enriched lymphocytes from mouse spleens and in the presence of a culture supernatant of WEHI-3 cells (WEHI-SUP), a population of cells expressing the BP-1 antigen appears progressively, reaches an optimal size 8 days after initiation of the culture, and disappears on day 28. In 8-day-old cultures, a minor population of cells bearing both BP-1 and B220 can be detected. The growth of this cell population, with characteristics of the B lymphoid lineage (pro-B), is strictly dependent on the presence of WEHI-SUP in the medium. After 2 weeks of culture, the BP-1 antigen is expressed on a cell population, which is essentially constituted of B220-, polynuclear cells. The BP-1 antigen, which is considered as characteristic of early cells of the B lymphoid lineage, can therefore also be expressed on cells of the myeloid lineage. The injection of BP-1+ or B220+ cells in irradiated mice can hardly reconstitute their B cell pool, whereas BP-1- and B220- cells are much more efficient in vivo progenitors of this cell lineage.
References
Jun 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·Y ReisnerN Sharon
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Q WuM D Cooper
Jun 25, 1985·Journal of Immunological Methods·R Chaby, R Girard
Oct 1, 1987·European Journal of Immunology·P L WitteP W Kincade
Jun 5, 1986·Nature·M D CooperP A Cazenave
Sep 1, 1986·Cellular Immunology·M BaccariniM L Lohmann-Matthes
Jan 1, 1987·Cell and Tissue Kinetics·R GirardR Chaby
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Oct 4, 1974·Nature·R A Phillips, R G Miller
Jan 1, 1983·Immunogenetics·C M HuangL A Herzenberg
May 1, 1984·Cellular Immunology·R ChabyR Girard
May 10, 1984·Nature·R PalaciosJ P McKearn
Jan 28, 1983·Journal of Immunological Methods·E Lamoyi, A Nisonoff
Mar 15, 1983·The Biochemical Journal·G W BazillT M Dexter
Mar 16, 1984·Journal of Immunological Methods·C A WhitlockO N Witte
Jan 1, 1982·Journal of Cellular Physiology. Supplement·N N IscoveL J Guilbert
Oct 1, 1980·The Journal of Experimental Medicine·T M DexterD Metcalf
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C A Whitlock, O N Witte
May 1, 1983·Immunology Today·D R ParksL A Herzenberg