PMID: 11911368Mar 26, 2002Paper

The spliced leader-associated RNA is a trypanosome-specific sn(o) RNA that has the potential to guide pseudouridine formation on the SL RNA

RNA
Xue-Hai LiangShulamit Michaeli

Abstract

The spliced leader-associated (SLA1) RNA is a trypanosome-specific small RNA with unknown function. SLA1 carries a Sm-like site, and is associated with core Sm proteins. Here we found that SLA1 belongs to a family of hairpin-containing RNAs that are implicated in directing pseudouridylation. A potential for base-pair interaction between SLA1 and spliced leader (SL) RNA agrees with the canonical rules for guiding pseudouridylation on SL RNA. Direct RNA analysis showed that this uridine is indeed pseudouridylated in the SL RNA of Leptomonas collosoma, Leishmania major, and Trypanosoma brucei. This position is conserved in all trypanosomatid SL RNAs. Mutations introduced in the SL RNA to disrupt the interaction domain of SLA1/SL RNA abolished the formation of the pseudouridine. SLA1 is localized both to the nucleolus and nucleoplasm. This study solves a long-standing question regarding the function of this novel RNA and describes the first H/ACA RNA, which, unlike all other pseudouridine guides, is also a bona fide snRNA.

References

Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·E Ullu, C Tschudi
Jun 29, 1990·Cell·N Agabian
Jun 11, 1990·Nucleic Acids Research·E Ullu, C Tschudi
Dec 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·K L PerryN Agabian
Jul 1, 1994·Molecular and Biochemical Parasitology·O BéjàS Michaeli
Dec 20, 1996·Molecular and Biochemical Parasitology·T G RobertsN Agabian
Jun 1, 1997·Current Opinion in Cell Biology·D Tollervey, T Kiss
May 30, 1997·Cell·C M Smith, J A Steitz
Aug 5, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y x XuS Michaeli
May 30, 1998·Nucleic Acids Research·A LevitanS Michaeli
Jun 20, 1998·Nucleic Acids Research·I GoncharovS Michaeli
Dec 9, 1998·Molecular Cell·K T TycowskiJ A Steitz
Aug 14, 1999·The Journal of Biological Chemistry·M N Schnare, M W Gray
Feb 24, 2000·Biochimica Et Biophysica Acta·M N Schnare, M W Gray
Jul 6, 2000·The Journal of Biological Chemistry·G MairC Tschudi
Jul 11, 2000·Molecular Biology of the Cell·T S Lange, S A Gerbi
Mar 30, 2001·The Journal of Biological Chemistry·Y XuS Michaeli
Aug 3, 2001·The Journal of Biological Chemistry·X H LiangS Michaeli

❮ Previous
Next ❯

Citations

Dec 16, 2006·Current Genetics·Andrea Hinas, Fredrik Söderbom
Jan 19, 2008·Theory in Biosciences = Theorie in Den Biowissenschaften·Athanasius F BompfünewererChristina Witwer
Nov 30, 2002·Biochimie·Jean Pierre BachellerieAlexander Hüttenhofer
Dec 10, 2002·Current Opinion in Chemical Biology·Alexander HüttenhoferJean Pierre Bachellerie
Apr 10, 2003·Eukaryotic Cell·Gusti M ZeinerDavid A Campbell
Oct 14, 2003·Eukaryotic Cell·Xue-hai LiangShulamit Michaeli
Feb 12, 2004·Eukaryotic Cell·Gusti M ZeinerDavid A Campbell
Dec 2, 2010·Microbiology and Molecular Biology Reviews : MMBR·Jan-Peter DanielsBill Wickstead
Oct 7, 2011·Nucleic Acids Research·Shulamit MichaeliElisabetta Ullu
Jan 19, 2008·Proteomics·Miguel A MoralesGerald F Späth
Mar 22, 2005·International Journal for Parasitology·Silvie Foldynová-TrantírkováJulius Lukes
Jun 15, 2004·Current Opinion in Structural Biology·Anthony K HenrasYves Henry
Jul 27, 2014·Current Opinion in Microbiology·Shulamit Michaeli
Aug 19, 2006·Molecular and Biochemical Parasitology·Moshe BitonShulamit Michaeli
Jun 24, 2009·Memórias do Instituto Oswaldo Cruz·Paola NocuaConcepción Puerta
Jul 25, 2003·The Journal of Biological Chemistry·Gusti M ZeinerDavid A Campbell
Feb 2, 2011·RNA Biology·Nicolas JaéAlbrecht Bindereif
Mar 3, 2004·The Journal of Biological Chemistry·Qing LiuShulamit Michaeli
Nov 15, 2002·The Journal of Biological Chemistry·Wayne A Decatur, Maurille J Fournier
Aug 24, 2019·The Journal of Biological Chemistry·Nikolay G KolevChristian Tschudi
Jan 4, 2019·Nucleic Acids Research·Vaibhav ChikneShulamit Michaeli

❮ Previous
Next ❯

Related Concepts

Related Feeds

African Trypanosomiasis

African trypanosomiasis, also known as sleeping sickness, is an insect-borne parasitic disease of humans and other animals. It is caused by protozoa of the species Trypanosoma brucei and almost invariably progresses to death unless treated. Discover the latest research on African trypanosomiasis here.