PMID: 3752542Jul 1, 1986

The split ejaculate: assessment of fertility potential using two in vitro test systems

Andrologia
R Z SokolR S Swerdloff

Abstract

Semen samples (split & whole ejaculates) were obtained from 12 normal men (group A) and 8 oligospermic infertile men with sperm concentrations of less than 20 x 10(6) sperm/ml (group B). All samples were evaluated by standard semen analysis, bovine cervical mucus penetration assay (CMPT), and, in all cases with sufficient sperm, in the human spermatozoa zona-free hamster in vitro penetration assay (SPA). In group A the motile sperm concentration was significantly higher in the ejaculated material of the first two contractions (fraction I or FI) than in the remainder of the ejaculate (fraction II or FII) (p less than 0.02). No significant differences were observed in sperm penetration into zona-free hamster ova or bovine cervical mucus by sperm from FI, FII or the whole ejaculate. Motile sperm concentration was significantly correlated with sperm penetration into bovine cervical mucus (r = 0.65, p less than 0.01), but not into zona-free hamster ova (r = 0.01 NS). In the samples collected by group B, the mean sperm concentration and motile sperm concentration were higher in the first (FI) than in the second (FII) fractions of the split ejaculate or the whole ejaculate (p less than 0.05). No significant differences were found amon...Continue Reading

References

Aug 1, 1979·Fertility and Sterility·R D NachtigallR H Glass
Dec 1, 1979·Fertility and Sterility·B J RogersR W Hale
Feb 1, 1977·Fertility and Sterility·R P Steiman, M L Taymor
Dec 15, 1977·American Journal of Obstetrics and Gynecology·K S MoghissiJ Yanez
Jun 11, 1976·Fertility and Sterility·R E DixonC W Schum
Apr 1, 1972·Fertility and Sterility·R Eliasson, C Lindholmer
Jun 1, 1983·International Journal of Andrology·R J AitkenM M Lees
Jul 1, 1983·Fertility and Sterility·D P WolfM M Quigley
Sep 1, 1983·Fertility and Sterility·E MargaliothJ G Schenker

Citations

Sep 21, 2016·Urologia·Kalanghot SkandhanVasudevan Jaya

Related Concepts

Palm-Leaf Reaction
Ejaculation
Artificial Insemination
Insemination, Artificial, Homologous
Oligospermia
Sperm Motility
Sperm-Zona Pellucida Penetration

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