The spot technique: synthesis and screening of peptide macroarrays on cellulose membranes

Methods in Molecular Biology
D Winkler, William D Campbell

Abstract

Peptide arrays are a widely used tool for drug development. For peptide-based drug design it is necessary to screen a large number of peptides. However, there are often difficulties with this approach. Most common peptide synthesis techniques are able to simultaneously synthesize only up to a few hundred single peptides. Spot synthesis is a positionally addressable, multiple synthesis technique offering the possibility of synthesizing and screening up to 10,000 peptides or peptide mixtures on cellulose or other membrane surfaces. In this chapter we present the basic procedures and screening methods related to spot synthesis and outline protocols for easy-to-use detection methods on these peptide arrays.

Citations

May 15, 2013·Expert Opinion on Drug Delivery·Saradha ChandrasekharVasant V Ranade
Apr 10, 2012·Bioscience, Biotechnology, and Biochemistry·Takashi OchiaiHiroyuki Honda
Feb 26, 2020·Proceedings of the National Academy of Sciences of the United States of America·Jiaming QiuJiang Xia
Jul 2, 2020·Cell Chemical Biology·Akul Y MehtaRichard D Cummings
Aug 27, 2014·Journal of the American Chemical Society·Mariya BarchAlan Jasanoff
Aug 29, 2021·Vaccines·Claudia GandolfoMaria Grazia Cusi
Feb 23, 2011·Biophysical Chemistry·Nils DrechslerRainer Rudolph

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