The SPR signal in living cells reflects changes other than the area of adhesion and the formation of cell constructions

Biosensors & Bioelectronics
Yuhki YanaseMichihiro Hide

Abstract

Surface plasmon resonance (SPR) sensors detected large angle of resonance (AR) changes, when RBL-2H3 rat mast cells were cultured and activated on a sensor chip. Here, we demonstrated that PAM212 mouse keratinocytes also showed a large change in AR, when EGF-stimulated. We explored these changes due to intracellular reactions, through the relationship between the AR and the area of cell adhesion, using confocal microscopy for RBL-2H3 cells and PAM212 cells. The effect of Mycalolide B and Toxin B, inhibitors for cell motility, on AR was observed using RBL-2H3 cells. Measuring AR in the presence of various numbers of non-stimulated cells demonstrated that AR and cell density were proportional. However, the AR increase in response to antigen was 35% higher than that expected by solely an increase of the cell adhesion area. Moreover, the AR with PAM212 cells decreased following a transient increase in response to EGF, whilst the area of cell adhesion remained at an increased level. Furthermore, the treatment of RBL-2H3 cells with either Mycalolide B or Toxin B slightly inhibited, but never abolished the AR increase induced by antigen. These treatments abolished all morphological changes, including ruffling and the increase of cell ...Continue Reading

References

Mar 13, 2003·Journal of Peptide Science : an Official Publication of the European Peptide Society·Henriette MozsolitsMarie-Isabel Aguilar
Jul 25, 2003·Analytical and Bioanalytical Chemistry·Jirí Homola

❮ Previous
Next ❯

Citations

May 31, 2012·Apoptosis : an International Journal on Programmed Cell Death·Jean-Sébastien MaltaisMichel Grandbois
Sep 11, 2013·Biosensors & Bioelectronics·Ivan StojanovićLeon W M M Terstappen
Feb 20, 2014·Biosensors & Bioelectronics·Yanina ShevchenkoAli Khademhosseini
Nov 28, 2013·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hongyan ZhangPengfei Wang
May 26, 2010·Immunology and Cell Biology·Yuhki YanaseNorio Sakai
Dec 12, 2012·Journal of Biomedical Optics·Victor YashunskyDan Davidov
Mar 13, 2014·Sensors·Yuhki YanaseMichihiro Hide
Oct 15, 2011·Cellular and Molecular Life Sciences : CMLS·Michael Saitakis, Electra Gizeli
Oct 11, 2008·Biosensors & Bioelectronics·Vincent ChabotPaul G Charette
Oct 28, 2008·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jun 18, 2015·Nanomedicine·Effat ZeidanMarinella G Sandros
Sep 18, 2008·Allergology International : Official Journal of the Japanese Society of Allergology·Hidenori SuzukiMichihiro Hide
Feb 26, 2013·Allergology International : Official Journal of the Japanese Society of Allergology·Yuhki YanaseMichihiro Hide
Jun 30, 2009·Biosensors & Bioelectronics·Sang Hun LeeTai Hyun Park
Feb 8, 2014·Analytical Chemistry·Pegah N AbadianEdgar D Goluch
Feb 19, 2015·Nanoscale·Musashi TakenakaAkihiko Kondo
Nov 2, 2018·Biosensors·Richard B M SchasfoortLeon W M M Terstappen
Apr 14, 2018·Analytical and Bioanalytical Chemistry·Yu-Wen Su, Wei Wang
Jun 7, 2014·Chemical Reviews·Qingjun LiuPing Wang
Dec 28, 2019·Analytical Chemistry·Hongwei CaiFeng Guo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.