The Src kinase Lck facilitates assembly of HIV-1 at the plasma membrane.

The Journal of Immunology : Official Journal of the American Association of Immunologists
Amy B StrasnerAndrew J Henderson

Abstract

HIV type 1 (HIV-1) assembly and egress are driven by the viral protein Gag and occur at the plasma membrane in T cells. Recent evidence indicates that secretory vesicles and machinery are essential components of virus packaging in both T cells and macrophages. However, the pathways and cellular mediators of Gag targeting to the plasma membrane are not well characterized. Lck, a lymphoid specific Src kinase critical for T cell activation, is found in the plasma membrane as well as various intracellular compartments and it has been suggested to influence HIV-1 replication. To investigate Lck as a potential regulator of Gag targeting, we assessed HIV-1 replication and Gag-induced virus-like particle release in the presence and absence of Lck. Release of HIV-1 and virus-like particles was reduced in the absence of Lck. This decrease in replication was not due to altered HIV-1 infection, transcription or protein translation. However, in T cells lacking Lck, HIV-1 accumulated intracellularly. In addition, expressing Lck in HeLa cells promoted HIV-1 Gag plasma membrane localization. Palmitoylation of the Lck unique domain, which is essential for directing Lck to the plasma membrane, was critical for its effect on HIV-1 replication. Fu...Continue Reading

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Citations

Mar 18, 2009·Immunological Reviews·Julie A ReadingerPamela L Schwartzberg
Jun 6, 2018·AIDS Research and Human Retroviruses·Luis M Agosto, Andrew J Henderson
Feb 7, 2014·The Journal of Biological Chemistry·Jiri VlachJamil S Saad
Dec 25, 2015·PloS One·Marta Martínez-BonetRicardo Madrid
Jun 17, 2016·Bioinformatics·Siddhartha JainZiv Bar-Joseph
Apr 7, 2010·The Journal of Biological Chemistry·Malini NatarajanAndrew J Henderson
Mar 10, 2011·Retrovirology·Elise Hamard-Peron, Delphine Muriaux
Apr 4, 2021·International Journal of Molecular Sciences·Charlotte BussienneSerena Bernacchi
Sep 6, 2011·Retrovirology·Charline GiroudLaurence Briant

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