PMID: 7542942Apr 1, 1995Paper

The SRP9/14 subunit of the signal recognition particle (SRP) is present in more than 20-fold excess over SRP in primate cells and exists primarily free but also in complex with small cytoplasmic Alu RNAs

Molecular Biology of the Cell
F BoviaK Strub

Abstract

The heterodimeric protein SRP9/14 bound to the Alu sequences of SRP RNA is essential for the translational control function of the signal recognition particle (SRP). The Alu RNAs of primate cells are believed to be derived from SRP RNA and have been shown to bind to an SRP14-related protein in vitro. We have used antibodies to characterize SRP9/14 and examine its association with small RNAs in vivo. Although SRP9 proteins are the same size in both rodent and primate cells, SRP14 subunits are generally larger in primate cells. An additional alanine-rich domain at the C-terminus accounts for the larger size of one human isoform. Although the other four SRP proteins are largely assembled into SRP in both rodent and primate cells, we found that the heterodimer SRP9/14 is present in 20-fold excess over SRP in primate cells. An increased synthesis rate of both proteins may contribute to their accumulation. The majority of the excess SRP9/14 is cytoplasmic and does not appear to be bound to any small RNAs; however, a significant fraction of a small cytoplasmic Alu RNA is complexed with SRP9/14 in a 8.5 S particle. Our findings that there is a large excess of SRP9/14 in primate cells and that Alu RNAs are bound to SRP9/14 in vivo sugge...Continue Reading

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Citations

Jan 1, 1995·Virus Genes·J Brosius, H Tiedge
Nov 30, 2000·Nucleic Acids Research·C AlemánP L Deininger
May 10, 2006·Nucleic Acids Research·Julien Häsler, Katharina Strub
Oct 6, 2006·Nucleic Acids Research·Julien Häsler, Katharina Strub
May 18, 2012·Journal of Virology·Sarra E Keene, Alice Telesnitsky
Aug 12, 2005·Cytogenetic and Genome Research·M Dewannieux, T Heidmann
Mar 8, 2013·PloS One·Yong-Feng RenKai-Fu Tang
May 14, 2005·Journal of Molecular Biology·Marie Dewannieux, Thierry Heidmann
Mar 19, 2014·Nucleus·Chen Wang, Sui Huang
Feb 6, 2013·Metabolic Engineering·Valérie Le FournNicolas Mermod
Dec 7, 2000·Genes, Chromosomes & Cancer·C M Rudin, C B Thompson
Aug 5, 2003·Nature Genetics·Marie DewannieuxThierry Heidmann
Jun 12, 2021·Frontiers in Molecular Biosciences·Camilla Faoro, Sandro F Ataide

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