The stage-regulated HASPB and SHERP proteins are essential for differentiation of the protozoan parasite Leishmania major in its sand fly vector, Phlebotomus papatasi.

Cellular Microbiology
Jovana SádlováDeborah F Smith

Abstract

The stage-regulated HASPB and SHERP proteins of Leishmania major are predominantly expressed in cultured metacyclic parasites that are competent for macrophage uptake and survival. The role of these proteins in parasite development in the sand fly vector has not been explored, however. Here, we confirm that expression of HASPB is detected only in vector metacyclic stages, correlating with the expression of metacyclic-specific lipophosphoglycan and providing the first definitive protein marker for this infective sand fly stage. Similarly, SHERP is expressed in vector metacyclics but is also detected at low levels in the preceding short promastigote stage. Using genetically modified parasites lacking or complemented for the LmcDNA16 locus on chromosome 23 that contains the HASP and SHERP genes, we further show that the presence of this locus is essential for parasite differentiation to the metacyclic stage in Phlebotomus papatasi. While wild-type and complemented parasites transform normally in late-stage infections, generating metacyclic promastigotes and colonizing the sand fly stomodeal valve, null parasites accumulate at the earlier elongated nectomonad stage of development within the abdominal and thoracic midgut of the sand...Continue Reading

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Citations

Dec 5, 2012·Parasites & Vectors·Anna Dostálová, Petr Volf
Sep 3, 2011·PLoS Neglected Tropical Diseases·Lisa W StamperNathan C Peters
Dec 30, 2011·PLoS Neglected Tropical Diseases·Ana Marta SilvaGraham H Coombs
Feb 1, 2012·PLoS Neglected Tropical Diseases·Diana MoreiraRicardo Silvestre
Jan 30, 2013·PLoS Neglected Tropical Diseases·Arie ZackayCharles L Jaffe
Dec 20, 2012·PloS One·Samuel O OyolaDeborah F Smith
Mar 4, 2014·PLoS Pathogens·Jessica L MartinNicola S Carter
Apr 25, 2013·Parasitology·Edward W TateMegan H Wright
Jan 20, 2012·Cellular Microbiology·Lorna M MacleanDeborah F Smith
Jun 25, 2013·Parasite Immunology·L MacLeanH P Price
Jul 8, 2015·Nucleic Acids Research·Laura A L DillonNajib M El-Sayed
Feb 2, 2015·Trends in Parasitology·Cinzia CantacessiDomenico Otranto
Apr 6, 2012·Vaccine·Bhawana Singh, Shyam Sundar
Jul 30, 2014·Parasitology·Andrew P Jackson
Feb 6, 2017·Molecular Aspects of Medicine·Mohammad AkhoundiDenis Sereno
Feb 25, 2015·Scientific Reports·Alejandro LlanesRicardo Lleonart
Feb 14, 2018·PloS One·Aygul IshemgulovaVyacheslav Yurchenko
Jul 13, 2011·Nature Reviews. Microbiology·Paul Kaye, Phillip Scott
May 10, 2019·PLoS Neglected Tropical Diseases·Pedro J AlcoleaVicente Larraga
Mar 4, 2020·PLoS Neglected Tropical Diseases·Iliano V Coutinho-AbreuJesus G Valenzuela
Aug 18, 2020·PLoS Neglected Tropical Diseases·Nathalia PinhoPatricia Cuervo
Nov 7, 2019·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Renata Cristina Ferreira DiasItalmar Teodorico Navarro
Feb 12, 2019·International Journal for Parasitology. Parasites and Wildlife·Jovana SadlovaPetr Volf
Mar 17, 2021·Virulence·Jovana SádlováNatalya Kraeva
May 13, 2021·Acta Tropica·Geovane Dias-LopesCarlos Roberto Alves

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Datasets Mentioned

BETA
AJ237587.1

Methods Mentioned

BETA
confocal microscopy
dissection
light microscopy
PCR
byacylation
transgenic

Software Mentioned

Image J
SPSS
Statistica

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