PMID: 1206576Nov 1, 1975Paper

The stages of osmotic haemolysis

The Journal of Physiology
A W Jay, S Rowlands

Abstract

The haemolysis of individual human erythrocytes has been observed using an inverted microscope and cine-camera. 2. With each permeant (glycerol, propylene glycol, ethylene glycol, urea and water) haemolysis is a multistage process. The stages are swelling, popping, reduction in volume possibly accompanied by ion leakage and, finally, haemoglobin leakage. 3. The classical haemolysis time (Th) is made up of a swelling time (Tsw) and a stress time (Tst). Tst is not negligible and with the faster permeants it may occupy more than 75% of the haemolysis time. 4. The stress time can also be divided into two parts: a K+ leak time (TK) during which the cell shrinks and a time (THb) during which haemoglobin is leaving the cell. THb occupies a substantial part of Th, from 25 to 65%, and is relatively longer in fast haemolysis. 5. There is a wide spread in the permeability coefficient to glycerol in a population of erythrocytes. The distribution is compatible with a Gaussian distribution. The mean permeability is 1-79 X 10(-6) cm/sec and the S.D. is +/0 0-45 X 10(-6) cm/sec. 6. The correlation between haemolysis time and swelling time for individual erythrocytes is poor, especially for fast haemolysis. Consequently, a measure of the distri...Continue Reading

Citations

Jan 1, 1981·The Journal of Membrane Biology·S Jausel-Hüsken, B Deuticke
Apr 25, 2007·Journal of Physiology and Biochemistry·H AhyayauchaM Bennouna
Sep 22, 2010·Journal of Bioenergetics and Biomembranes·L C FonsecaN Penha-Silva
Jun 1, 1989·Archives internationales de physiologie et de biochimie·R E SerraniJ L Corchs
Feb 26, 2013·Biotechnology Progress·Ratih E LusiantiAdam Z Higgins
Jan 1, 1983·Journal of Biomechanics·H A MassaldiC H Borzi
Feb 21, 1984·Journal of Theoretical Biology·H A Massaldi, C H Borzi
Oct 1, 1988·Burns, Including Thermal Injury·Y YuanZ H Zhang
Sep 24, 2005·Lasers in Surgery and Medicine·Serge MordonJean Claude Lesage
Nov 6, 2018·Forensic Science, Medicine, and Pathology·Tomoya IkedaTakaki Ishikawa
Mar 24, 1999·Molecular Membrane Biology·M Baumann, R Grebe
Dec 1, 1982·Cell Biophysics·L S SewchandS Rowlands
Jan 1, 1981·Upsala Journal of Medical Sciences·N V MarsdenH G Davies
May 30, 2019·Scientific Reports·Snigdha SinghAditya Mittal
Mar 1, 1980·The American Journal of Physiology·R A GarrickF P Chinard
Apr 12, 1995·Biochimica Et Biophysica Acta·S V Rudenko, S V Patelaros
Jul 6, 1981·Biochimica Et Biophysica Acta·K Araki, J M Rifkind
Jan 1, 1990·Comparative Biochemistry and Physiology. A, Comparative Physiology·H Ginsberg
Nov 3, 1988·Biochimica Et Biophysica Acta·M MosiorJ Gomułkiewicz

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.