PMID: 9644817Jun 30, 1998Paper

The staining of the central neurons in the pteropod mollusk Clione limacina with fluorescein-labelled alpha-bungarotoxin

Zhurnal vyssheĭ nervnoĭ deiatelnosti imeni I P Pavlova
L B Popova, Iu V Panchin

Abstract

FITC-labelled alpha-bungarotoxin (alpha BT[FITC]) was used to identify acetylcholine (ACh)-sensitive neurons in the central nervous system of pteropod mollusc Clione limacina. Two small symmetrical neurons were stained with alpha BT[FITC] on the dorsal surface of the cerebral ganglia. The staining was highly specific as it was completely blocked by preincubation in alpha-bungarotoxin [alpha BT] at very low concentration (10(-9) M). Nicotinic agonists and antagonists efficiently prevented alpha BT[FITC] staining while muscarinic agonists and antagonists had no effect. These findings confirm the suggestion that alpha BT[FITC] binds to nicotinic ACh receptors. The possible role is discussed of alpha BT[FITC]-stained cells in the complex of hunting behavior of Clione.

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