The status of donor cancer tissues affects the fate of patient-derived colorectal cancer xenografts in NOG mice

Experimental Animals
Etsuko FujiiMasami Suzuki

Abstract

Patient-derived xenografts (PDXs) of tumors are increasingly becoming important tools for translational research in oncology. The NOD.Cg-Prkdc(scid) Il2rg(tm1Sug)/Jic (NOG) mouse is an efficient host for PDXs. Thus as a basis for future development of methods to obtain PDXs from various disease types, we have studied the factors that affect the outcome of transplantation of human colorectal cancer in NOG mice. Of the original donor cases examined, 73% had successful engraftment. The outcome of donor-matched tissues was consistent in most cases, and was thought to show that the condition of the host did not affect engraftment. Next we analyzed the tumor aggressiveness in terms of histology grade of the original tumor and found that they were related to engraftment. Detailed histopathological examination of the transplanted tissues strongly indicated that lymphocytes engrafted with the tumor cells affect engraftment. As a factor related to transplantation of lymphocytes, we studied the human IgG concentration in the serum of tumor-bearing mice, but there was no tendency for higher concentrations to result in unsuccessful engraftment. Finally, we studied the type, density and location of T cells in the original donor tissue to det...Continue Reading

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Citations

Aug 8, 2021·Journal of Experimental & Clinical Cancer Research : CR·Daniel StrüderClaudia Maletzki

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Methods Mentioned

BETA
xenografts
xenograft
ELISA

Software Mentioned

Magellan Data Reduction
Image Scope

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