The steady-state level and stability of TLS polymerase eta are cell cycle dependent in the yeast S. cerevisiae

DNA Repair
Michal PlachtaEwa Sledziewska-Gojska

Abstract

Polymerase eta (Pol eta) is a ubiquitous translesion DNA polymerase that is capable of bypassing UV-induced pyrimidine dimers in an error-free manner. However, this specialized polymerase is error prone when synthesizing through an undamaged DNA template. In Saccharomyces cerevisiae, both depletion and overproduction of Pol eta result in mutator phenotypes. Therefore, regulation of the cellular abundance of this enzyme is of particular interest. However, based on the investigation of variously tagged forms of Pol eta, mutually contradictory conclusions have been reached regarding the stability of this polymerase in yeast. Here, we optimized a protocol for the detection of untagged yeast Pol eta and established that the half-life of the native enzyme is 80 ± 14 min in asynchronously growing cultures. Experiments with synchronized cells indicated that the cellular abundance of this translesion polymerase changes throughout the cell cycle. Accordingly, we show that the stability of Pol eta, but not its mRNA level, is cell cycle stage dependent. The half-life of the polymerase is more than fourfold shorter in G1-arrested cells than in those at G2/M. Our results, in concert with previous data for Rev1, indicate that cell cycle regul...Continue Reading

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Citations

Jun 1, 2016·Frontiers in Genetics·Néstor García-RodríguezHelle D Ulrich
Dec 25, 2015·Cell Cycle·Irene GallinaVibe H Oestergaard
Sep 22, 2017·Nucleic Acids Research·Federica BertolettiSimone Sabbioneda

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