The Stealthy Superbug: the Role of Asymptomatic Enteric Carriage in Maintaining a Long-Term Hospital Outbreak of ST228 Methicillin-Resistant Staphylococcus aureus

MBio
Laurence SennDominique S Blanc

Abstract

Whole-genome sequencing (WGS) of 228 isolates was used to elucidate the origin and dynamics of a long-term outbreak of methicillin-resistant Staphylococcus aureus (MRSA) sequence type 228 (ST228) SCCmec I that involved 1,600 patients in a tertiary care hospital between 2008 and 2012. Combining of the sequence data with detailed metadata on patient admission and movement confirmed that the outbreak was due to the transmission of a single clonal variant of ST228, rather than repeated introductions of this clone into the hospital. We note that this clone is significantly more frequently recovered from groin and rectal swabs than other clones (P < 0.0001) and is also significantly more transmissible between roommates (P < 0.01). Unrecognized MRSA carriers, together with movements of patients within the hospital, also seem to have played a major role. These atypical colonization and transmission dynamics can help explain how the outbreak was maintained over the long term. This "stealthy" asymptomatic colonization of the gut, combined with heightened transmissibility (potentially reflecting a role for environmental reservoirs), means the dynamics of this outbreak share some properties with enteric pathogens such as vancomycin-resista...Continue Reading

References

Apr 7, 1999·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R M AnthonyG L French
Dec 10, 1999·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D S BlancP Francioli
Apr 10, 2002·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A S Rossney, C T Keane
Sep 25, 2002·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Cheryl SquierNina Singh
Nov 1, 2002·American Journal of Infection Control·Luciano SilvestriHendrick K F van Saene
Oct 9, 2004·Journal of Clinical Microbiology·Eduardo Pérez-RothSebastián Méndez-Alvarez
May 4, 2005·Journal of Bacteriology·Anne-Laure Prunier, Roland Leclercq
Sep 29, 2006·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Ralf-Peter VonbergPetra Gastmeier
Aug 19, 2007·The Journal of Hospital Infection·John M Boyce
Sep 11, 2007·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·John M BoyceNicholas M T Adams
May 13, 2008·The Lancet Infectious Diseases·Werner C Albrich, Stephan Harbarth
May 27, 2008·Intensive Care Medicine·Rahul BatraJonathan Edgeworth
May 31, 2008·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·M BuehlmannA F Widmer
Jun 19, 2008·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M HallinM J Struelens
Jul 11, 2008·Journal of Clinical Microbiology·Marta Aires-de-SousaUNKNOWN Multilaboratory Project Collaborators
Aug 15, 2009·Nature Reviews. Microbiology·Henry F Chambers, Frank R Deleo
Dec 17, 2009·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rahul BatraJonathan D Edgeworth
Jan 20, 2010·PLoS Medicine·Hajo GrundmannUNKNOWN European Staphylococcal Reference Laboratory Working Group
Jan 23, 2010·Science·Simon R HarrisStephen D Bentley
May 16, 2012·Proceedings of the National Academy of Sciences of the United States of America·Paul R McAdamJ Ross Fitzgerald
Jun 15, 2012·The New England Journal of Medicine·Claudio U KöserSharon J Peacock
Apr 23, 2014·Proceedings of the National Academy of Sciences of the United States of America·Anne-Catrin UhlemannFranklin D Lowy
Jul 5, 2015·The Journal of Antimicrobial Chemotherapy·T PoovelikunnelH Humphreys

❮ Previous
Next ❯

Citations

Jun 1, 2016·Frontiers in Microbiology·Shantelle Claassen-WeitzMamadou Kaba
Oct 22, 2016·Annals of the New York Academy of Sciences·Michael R GillingsSasha G Tetu
Feb 6, 2017·American Journal of Infection Control·Patrick TangLinda M Hoang
May 19, 2017·Current Opinion in Infectious Diseases·Hasan M Al-Dorzi, Yaseen M Arabi
Jul 21, 2017·Expert Review of Anti-infective Therapy·Julie GagnaireElisabeth Botelho-Nevers
Feb 26, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Isaac SeeAlexander J Kallen
Oct 12, 2018·Nature·Pipat PiewngamMichael Otto
Jan 9, 2020·Microbial Genomics·Stefano G GiulieriDeborah A Williamson
Aug 16, 2019·Sports Medicine - Open·Jamie E DeNizio, David A Hewitt
Oct 1, 2017·Scientific Reports·Amy FlaxmanDavid H Wyllie
May 5, 2018·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Marco CassoneLona Mody
Mar 28, 2019·Gut Microbes·Pipat Piewngam, Michael Otto
Apr 4, 2017·FEMS Microbiology Reviews·François DelavatJan Roelof van der Meer
Dec 7, 2018·BMC Microbiology·Timothy D ReadMichael Z David
Dec 5, 2018·Frontiers in Microbiology·Patrick T LaBreckD Scott Merrell
Jul 6, 2019·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Julie GagnairePaul O Verhoeven
Apr 18, 2018·Frontiers in Microbiology·Emilie L FisherGordon Y C Cheung
Aug 3, 2016·Annual Review of Microbiology·J Ross Fitzgerald, Matthew T G Holden
Nov 6, 2020·International Journal of Environmental Research and Public Health·Martyna KaselaAnna Malm
Feb 2, 2021·Virulence·Gordon Y C CheungMichael Otto
Nov 18, 2020·Genome Medicine·Ana Berbel CabanHarm van Bakel
Jun 24, 2021·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Maria A ArgudínMarie Hallin
Jul 15, 2021·FEMS Microbiology Reviews·Elisa J M RaineriJan Maarten van Dijl
Jun 3, 2021·Journal of Clinical Microbiology·Alison Laufer HalpinChristopher A Elkins

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR

Software Mentioned

NetDraw
MATLAB

Related Concepts

Related Feeds

CRISPR & Staphylococcus

CRISPR-Cas system enables the editing of genes to create or correct mutations. Staphylococci are associated with life-threatening infections in hospitals, as well as the community. Here is the latest research on how CRISPR-Cas system can be used for treatment of Staphylococcal infections.